DETERMINATION OF THE DETECTION LIMIT OF THE POLYMERASE CHAIN-REACTION FOR CHICKEN INFECTIOUS-ANEMIA VIRUS

被引：38

作者：

SOINE, C

WATSON, SK

RYBICKI, E

LUCIO, B

NORDGREN, RM

PARRISH, CR

SCHAT, KA

机构：

[1] CORNELL UNIV,COLL VET MED,DEPT AVIAN & AQUAT ANIM MED,ITHACA,NY 14853

[2] BOYCE THOMPSON INST PLANT RES,ITHACA,NY 14850

[3] SOLVAY ANIM HLTH,MENDOTA HTS,MN 55120

[4] CORNELL UNIV,COLL VET MED,JAMES A BAKER INST ANIM HLTH,ITHACA,NY 14853

来源：

AVIAN DISEASES | 1993年 / 37卷 / 02期

关键词：

D O I：

10.2307/1591674

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Chicken infectious anemia virus (CIAV) DNA in infected cell cultures and chicken tissues was detected using a polymerase chain reaction (PCR) assay. The complete CIAV genome of several strains was amplified in two segments with two sets of primer pairs. The DNA segments of four CIAV strains and full-length Cux-1 strain DNA were cloned. After amplification, 100 original genome equivalents were detected by Southern hybridization. The sensitivity of the assay was enhanced considerably by performing a reamplification with nested primers. This modification permitted the detection of one molecule of CIAV DNA. Some problems of the assay and its possible application are discussed.

引用

页码：467 / 476

页数：10

共 28 条

[1] ISOLATION AND IDENTIFICATION OF CHICKEN INFECTIOUS-ANEMIA VIRUS IN BRAZIL [J].