IMPROVEMENT OF LATERAL RESOLUTION IN FAR-FIELD FLUORESCENCE LIGHT-MICROSCOPY BY USING 2-PHOTON EXCITATION WITH OFFSET BEAMS

被引：62

作者：

HELL, SW ^{[1
]}

机构：

[1] UNIV TURKU,CTR BIOTECHNOL,SF-20521 TURKU,FINLAND

来源：

OPTICS COMMUNICATIONS | 1994年 / 106卷 / 1-3期

关键词：

Focusing - Light polarization - Microscopic examination - Numerical analysis - Photons;

D O I：

10.1016/0030-4018(94)90050-7

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A method is described of increasing the resolution in far-field fluorescence light microscopy by a factor of 2. Calculations show that a lateral resolution of 75 nm is achieved with a lens of numerical aperture of 1.4 (oil immersion) by a two-photon excitation mode with each photon stemming from displaced focused light beams of lambda=500 nm and overlapping main maxima.

引用

页码：19 / 24

页数：6

共 9 条

[1] DARNELL J, 1990, MOL CELL BIOL, P117
[2] ERMOLAEV VL, 1984, OPT SPEKTROSK+, V56, P1026
[3] PROPERTIES OF A 4PI CONFOCAL FLUORESCENCE MICROSCOPE
HELL, S
STELZER, EHK
[J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1992, 9 (12) : 2159 - 2166
[4] FUNDAMENTAL IMPROVEMENT OF RESOLUTION WITH A 4PI-CONFOCAL FLUORESCENCE MICROSCOPE USING 2-PHOTON EXCITATION
HELL, S
STELZER, EHK
[J]. OPTICS COMMUNICATIONS, 1992, 93 (5-6) : 277 - 282
[5] HELL SW, IN PRESS APPL PHYS L
[6] HELL SW, 1992, Patent No. 911213684
[7] ISAACSON M, 1990, ULTRAMICROSCOPY, V47, P15
[8] Richards B., 1959, P ROY SOC LOND A MAT, V253, P349
[9] SARIKAYA M, 1990, ULTRAMICROSCOPY, V47, P1

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