EPITOPE MAPPING OF CROSS-REACTIVE MONOCLONAL-ANTIBODIES SPECIFIC FOR THE INFLUENZA-A VIRUS PA AND PB2 POLYPEPTIDES

被引：22

作者：

OCHOA, M

BARCENA, J

DELALUNA, S

MELERO, JA

DOUGLAS, AR

NIETO, A

ORTIN, J

SKEHEL, JJ

PORTELA, A

机构：

[1] CTR NACL BIOL CELULAR & RETROVIRUS,INST SALUD CARLOS 3,E-28220 MADRID,SPAIN

[2] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN

[3] NATL INST MED RES,LONDON NW7 1AA,ENGLAND

来源：

VIRUS RESEARCH | 1995年 / 37卷 / 03期

关键词：

INFLUENZA VIRUS; MONOCLONAL ANTIBODY; POLYMERASE; ANTIGENIC VARIATION;

D O I：

10.1016/0168-1702(95)00039-S

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Barcena et al. (1994) J. Virol. 68, 6900-6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three non-overlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1-113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.

引用

页码：305 / 315

页数：11

共 28 条

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