CHLAMYDIAL LIPOPOLYSACCHARIDE - CHEMICAL AND ANTIGENIC STRUCTURE, BIOSYNTHESIS AND BIOMEDICAL APPLICATION

被引：8

作者：

BRADE, H ^{[1
]}

BRABETZ, W ^{[1
]}

BRADE, L ^{[1
]}

HOLST, O ^{[1
]}

LOBAU, S ^{[1
]}

LUKACOVA, M ^{[1
]}

MAMAT, U ^{[1
]}

ROZALSKI, A ^{[1
]}

ZYCH, K ^{[1
]}

KOSMA, P ^{[1
]}

机构：

[1] AGR UNIV VIENNA, INST CHEM, A-1180 VIENNA, AUSTRIA

来源：

PURE AND APPLIED CHEMISTRY | 1995年 / 67卷 / 10期

关键词：

D O I：

10.1351/pac199567101617

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The obligate intracellular gram-negative bacterium Chlamydia contains as a major surface antigen a lipopolysaccharide (LPS) which harbours in its saccharide moiety a genus-specific epitope composed of a linear trisaccharide of 3-deoxy-D-manno-octulopyranosonic acid (Kdo) of the sequence alpha Kdo-(2->8)-cuKdo-(2->4)-alpha Kdo. The structure was established on LPS of recombinant E. coli bacteria transformed with a plasmid carrying the gene for the chlamydial Kdo transferase which is a multifunctional glycosyl transferase. The structure was determined on deacylated and dephosphorylated LPS as well as on deacylated LPS by qualitative, quantitative and methylation analyses, fast-atom-bombardment mass spectrometry, and H-1-,C-13, and P-31-NMR spectroscopy, and confirmed by chemcial synthesis. Artificial glycoconjugate antigens were synthesized and used for the preparation of murine monoclonal antibodies which had higher affinities than those prepared against the natural counterpart. These artificial antigens were also used as solid-phase antigens in an enzyme-immune assay which was proven to be useful in the diagnosis of human chlamydial infections.

引用

页码：1617 / 1626

页数：10

共 69 条

[1] STRUCTURE OF THE K74 ANTIGEN FROM ESCHERICHIA-COLI O44-K74-H18, A CAPSULAR POLYSACCHARIDE CONTAINING FURANOSIDIC BETA-KDO RESIDUES [J].