THYMIDINE-5'-TRIPHOSPHATE INCORPORATION INTO DNA BY RAT THYMUS NUCLEI

被引：17

作者：

LAGUNOFF, D

机构：

[1] Department of Pathology, University of Washington, Seattle

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 55卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0014-4827(69)90454-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

It has been possible to isolate rat thymus nuclei that retain the capacity to incorporate thymidine-5′-triphosphate into DNA bound to the nucleus. The reaction is stimulated by the presence of the other three deoxynucleoside-5′-triphosphates, Mg2+ and an excess of ATP. Both denatured and native DNA, when added to the nuclei, serve as effective templates. In those preparations with least contamination with intact cells, thymidine-5′-triphosphate is incorporated at a more rapid rate than thymidine. © 1969.

引用

页码：53 / &

共 16 条

[1] BAZILL GW, 1963, BIOCHIM BIOPHYS ACTA, V76, P223
[2] BILLEN D, 1963, BIOCHIM BIOPHYS ACTA, V68, P342
[3] NUCLEI FROM RAT LIVER - ISOLATION METHOD THAT COMBINES PURITY WITH HIGH YIELD
BLOBEL, G
POTTER, VR
[J]. SCIENCE, 1966, 154 (3757) : 1662 - &
[4] BOLLUM FJ, 1959, CANCER RES, V19, P561
[5] BOLLUM FJ, 1958, J BIOL CHEM, V233, P478
[6] BOLLUM JF, 1966, PROCEDURES NUCLEIC A, P296
[7] BUTTIN G, 1966, J BIOL CHEM, V241, P5419
[8] CANTONI GL, 1966, PROCEDURES NUCLEI ED, P296
[9] FLECK A, 1962, BIOCHIM BIOPHYS ACTA, V55, P571
[10] FRIEDKIN N, 1956, J BIOL CHEM, V220, P639

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