LOSS OF RESPIRATORY ENZYME-ACTIVITY IN ANOXIC MYOCARDIUM EFFECT OF PROPRANOLOL

被引:4
作者
CARROLL, BJ
WELMAN, E
机构
[1] Cardiovascular Research Unit, Royal Postgraduate Medical School, London
关键词
Anoxia; Creatine kinase; Cytochrome oxidase; Guinea pig; Mitochondria; propranolol; Succinate dehydrogenase;
D O I
10.1016/0022-2828(79)90001-4
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Guinea pig hearts were perfused anoxically with and without dl-propranolol hydrochloride (0.1 mg.l-1) to determine whether a therapeutic level of this drug could protect the inner mitochondrial membrane. After 5 h anoxia, the specific activities of the mitochondrial inner membrane enzymes, succinate dehydrogenase and cytochrome oxidase, were reduced to approximately half of the activities before perfusion. The activity of the mainly cytosolic enzyme, creatine kinase, was reduced by 58% during 5 h anoxia. In the presence of propranolol, the loss of mitochondrial enzyme activity was significantly reduced, while the loss of creatine kinase activity was unaltered. These findings indicate (1) that loss of respiratory enzyme activity is a consequence of irreversible cellular damage due to anoxia, (2) that a therapeutic level of propranolol conserves respiratory enzyme activity and (3) that the protective action of this level of propranolol is insufficient to conserve creatine kinase activity. © 1979.
引用
收藏
页码:1209 / 1214
页数:6
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