INVITRO PACKAGING OF THE BACTERIOPHAGE-PHI-6 SSRNA GENOMIC PRECURSORS

被引：51

作者：

GOTTLIEB, P ^{[1
]}

STRASSMAN, J ^{[1
]}

FRUCHT, A ^{[1
]}

QIAO, XY ^{[1
]}

MINDICH, L ^{[1
]}

机构：

[1] PUBL HLTH RES INST CITY NEW YORK INC, DEPT MICROBIOL, 455 1ST AVE, NEW YORK, NY 10016 USA

来源：

VIROLOGY | 1991年 / 181卷 / 02期

关键词：

D O I：

10.1016/0042-6822(91)90892-F

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteriophage φ6 contains three segments of double-stranded RNA within a nucleocapsid. Plasmids containing cDNA copies of the large genomic segment direct the synthesis of viral proteins that assemble into procapsids in Escherichia coli or Pseudomonas phaseolicola. These structures are dodecahedral assemblages of proteins P1, P2, P4, and P7. We report in this paper that these particles are capable of packaging viral single-stranded plus-sense RNA in vitro. The packaging reaction requires the presence of ATP or dATP. Synthesis of minus strands takes place within this filled procapsid in the presence of all four nucleoside triphosphates. Packaged ssRNA is found to be protected from added ribonuclease. © 1991.

引用

页码：589 / 594

页数：6

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