PROTECTION OF COTTON RATS BY IMMUNIZATION WITH THE HUMAN PARAINFLUENZA VIRUS TYPE-3 FUSION (F) GLYCOPROTEIN EXPRESSED ON THE SURFACE OF INSECT CELLS INFECTED WITH A RECOMBINANT BACULOVIRUS

被引：18

作者：

HALL, SL

MURPHY, BR

COELINGH, KLV

机构：

[1] Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda

来源：

VACCINE | 1991年 / 9卷 / 09期

关键词：

PIV3 FUSION GLYCOPROTEIN; BACULOVIRUS VECTOR; IMMUNIZATION;

D O I：

10.1016/0264-410X(91)90192-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The antigenicity, immunogenicity and efficacy of the human PIV3 fusion (F) glycoprotein expressed in insect cells by a baculovirus vector were studied. The results indicate that the PIV3 F glycoprotein expressed by a recombinant baculovirus is antigenically authentic as determined using a panel of PIV3 F specific monoclonal antibodies. Only a low level of antibody was stimulated by immunization of animals with infected cells, but the antibody appeared to be of high quality. Immunized animals were also moderately protected against PIV3 challenge. These results indicate that the baculovirus expression system is a reasonable source of authentic PIV3 F protein for use in a subunit vaccine.

引用

页码：659 / 667

页数：9

共 36 条

[1] NEUTRALIZATION EPITOPES OF THE F-GLYCOPROTEIN OF RESPIRATORY SYNCYTIAL VIRUS - EFFECT OF MUTATION UPON FUSION FUNCTION [J].

BEELER, JA ;

COELINGH, KV .

JOURNAL OF VIROLOGY, 1989, 63 (07) :2941-2950

[2]

BUSER F, 1970, Scandinavian Journal of Infectious Diseases, V2, P157, DOI 10.3109/inf.1970.2.issue-3.01

[3] CONSERVED EPITOPES ON THE HEMAGGLUTININ-NEURAMINIDASE PROTEINS OF HUMAN AND BOVINE PARA-INFLUENZA TYPE-3 VIRUSES - NUCLEOTIDE-SEQUENCE ANALYSIS OF VARIANTS SELECTED WITH MONOCLONAL-ANTIBODIES [J].

COELINGH, KJ ;

WINTER, CC ;

MURPHY, BR ;

RICE, JM ;

KIMBALL, PC ;

OLMSTED, RA ;

COLLINS, PL .

JOURNAL OF VIROLOGY, 1986, 60 (01) :90-96

[4] ANTIGENIC VARIATION IN THE HEMAGGLUTININ-NEURAMINIDASE PROTEIN OF HUMAN PARA-INFLUENZA TYPE-3 VIRUS [J].

COELINGH, KLV ;

WINTER, C ;

MURPHY, BR .

VIROLOGY, 1985, 143 (02) :569-582

[5] ANTIBODY-RESPONSES OF HUMANS AND NONHUMAN-PRIMATES TO INDIVIDUAL ANTIGENIC SITES OF THE HEMAGGLUTININ-NEURAMINIDASE AND FUSION GLYCOPROTEINS AFTER PRIMARY INFECTION OR REINFECTION WITH PARAINFLUENZA TYPE-3 VIRUS [J].

COELINGH, KLV ;

WINTER, CC ;

TIERNEY, EL ;

HALL, SL ;

LONDON, WT ;

KIM, HW ;

CHANOCK, RM ;

MURPHY, BR .

JOURNAL OF VIROLOGY, 1990, 64 (08) :3833-3843

[6] NUCLEOTIDE AND DEDUCED AMINO-ACID SEQUENCE OF HEMAGGLUTININ-NEURAMINIDASE GENES OF HUMAN TYPE-3 PARA-INFLUENZA VIRUSES ISOLATED FROM 1957 TO 1983 [J].

COELINGH, KLV ;

WINTER, CC ;

MURPHY, BR .

VIROLOGY, 1988, 162 (01) :137-143

[7] EXPRESSION OF BIOLOGICALLY-ACTIVE AND ANTIGENICALLY AUTHENTIC PARA-INFLUENZA TYPE-3 VIRUS HEMAGGLUTININ-NEURAMINIDASE GLYCOPROTEIN BY A RECOMBINANT BACULOVIRUS [J].

COELINGH, KLV ;

MURPHY, BR ;

COLLINS, PL ;

LEBACQVERHEYDEN, AM ;

BATTEY, JF .

VIROLOGY, 1987, 160 (02) :465-472

[8] IDENTIFICATION OF AMINO-ACIDS RECOGNIZED BY SYNCYTIUM-INHIBITING AND NEUTRALIZING MONOCLONAL-ANTIBODIES TO THE HUMAN PARA-INFLUENZA TYPE-3 VIRUS FUSION PROTEIN [J].

COELINGH, KV ;

TIERNEY, EL .

JOURNAL OF VIROLOGY, 1989, 63 (09) :3755-3760

[9] ANTIGENIC AND FUNCTIONAL-ORGANIZATION OF HUMAN PARA-INFLUENZA VIRUS TYPE-3 FUSION GLYCOPROTEIN [J].

COELINGH, KV ;

TIERNEY, EL .

JOURNAL OF VIROLOGY, 1989, 63 (01) :375-382

[10] PARAINFLUENZA VIRUS IMMUNIZATION .2. INFLUENCE OF AGE AND MATERNAL ANTIBODY UPON SUCCESSFUL IMMUNIZATION WITH AN ALUM-ADSORBED PARAINFLUENZA TYPE 3 VACCINE [J].

FULGINITI, VA ;

SIEBER, OF ;

JOHN, TJ ;

ASKIN, P ;

UMLAUF, HJ .

PEDIATRIC RESEARCH, 1967, 1 (01) :50-+

← 1 2 3 4 →