PEROXIDE-DEPENDENT AND PEROXIDE-INDEPENDENT LIPID PEROXIDATIONS CATALYZED BY CHELATED IRON

被引:24
作者
FUKUZAWA, K [1 ]
FUJII, T [1 ]
MUKAI, K [1 ]
机构
[1] EHIME UNIV,FAC SCI,DEPT CHEM,MATSUYAMA,EHIME 790,JAPAN
关键词
D O I
10.1016/0003-9861(91)90571-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oxidation of linoleic acid (LA) in tetradecyltrimethyl-ammonium bromide micelles was induced by ferrous- and ferric-chelates in the presence of linoleic acid hydroperoxide (LOOH). Ferrous-chelates also induced lipid peroxidation in the presence of H2O2, but ferric-chelates did not, though they could generate OH-radicals in the presence of H2O2, resulting in deoxyribose degradation. Of the chelators tested, nitrilotriacetic acid (NTA) chelated with iron showed the highest activity for inductions of H2O2- and LOOH-dependent lipid peroxidations and H2O2-dependent deoxyribose degradation. NTA with ferrous ion, but not with ferric ion, also initiated oxidation of LA after a short lag period in the absence of peroxides such as H2O2 and LOOH, but other chelators with ferrous ion did not. The peroxide-independent lipid peroxidation and associated oxidation of ferrous-NTA to ferric-NTA progressed in two steps: an induction step in a lag period and then a propagation step. Ferrous ion complexed with NTA was autoxidized pH-dependently and synchronously with oxygen uptake. The rates of both reactions increased with increase of pH, but were not related to the length of the lag period, which was also dependent on pH, and was shortest at pH 4.2. The EPR spectrum of the ferric-NTA complex prepared directly from ferric salt was different from that of the complex prepared from ferrous salt, confirming that some ferric-type active oxygen participated in induction of peroxide-independent lipid peroxidation. From these results, we propose a possible mechanism of lipid peroxidation induced by ferrous-NTA without peroxides. The finding that iron-NTA had the highest activity for induction of the oxidations of LA and deoxyribose is discussed in relation to the carcinogenic and nephrotoxic effects of this chelating agent. © 1991.
引用
收藏
页码:489 / 496
页数:8
相关论文
共 31 条
[1]  
ARUOMA OI, 1989, J BIOL CHEM, V264, P20509
[2]  
BRAUGHLER JM, 1986, J BIOL CHEM, V261, P282
[3]  
BUCHER JR, 1983, OXY RADICALS THEIR S, V1, P292
[4]  
Buege J A, 1978, Methods Enzymol, V52, P302
[5]  
DAVIS JT, 1963, INTERFACIAL PHENOMEN, P95
[6]  
EBINA Y, 1986, J NATL CANCER I, V76, P107
[7]   LIPID-PEROXIDATION OF RABBIT SMALL INTESTINAL MICROVILLUS MEMBRANE-VESICLES BY IRON COMPLEXES [J].
FODOR, I ;
MARX, JJM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 961 (01) :96-102
[8]   SITE-SPECIFIC MECHANISMS OF INITIATION BY CHELATED IRON AND INHIBITION BY ALPHA-TOCOPHEROL OF LIPID PEROXIDE-DEPENDENT LIPID-PEROXIDATION IN CHARGED MICELLES [J].
FUJII, T ;
HIRAMOTO, Y ;
TERAO, J ;
FUKUZAWA, K .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 284 (01) :120-126
[9]   SITE-SPECIFIC INDUCTION OF LIPID-PEROXIDATION BY IRON IN CHARGED MICELLES [J].
FUKUZAWA, K ;
TADOKORO, T ;
KISHIKAWA, K ;
MUKAI, K ;
GEBICKI, JM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 260 (01) :146-152
[10]  
FUKUZAWA K, 1989, MED BIOCH CHEM ASPEC, V1, P441