STABLE EXPRESSION AND REGULATION OF A RAT-BRAIN K+ CHANNEL

被引：61

作者：

CRITZ, SD

WIBLE, BA

LOPEZ, HS

BROWN, AM

机构：

[1] Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas

来源：

JOURNAL OF NEUROCHEMISTRY | 1993年 / 60卷 / 03期

关键词：

NGK2; CHANNELS; PERMEATION; TRANSFECTION; HEK; 293; CELLS; KV3.1;

D O I：

10.1111/j.1471-4159.1993.tb03273.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Shaw-type K+ channel K(v)3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to K(v)3.1 channels expressed transiently in Xenopus oocytes. K(v)3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased K(v)3.1 currents. Based on these results, we find that the stable expression of voltage-gated K+ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.

引用

页码：1175 / 1178

页数：4

共 19 条

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