ALLOZYME COMPARISON OF 3 TRYPANOSOMA SPECIES (KINETOPLASTIDA, TRYPANOSOMATIDAE) OF TOADS AND FROGS BY STARCH-GEL ELECTROPHORESIS

被引:14
作者
MARTIN, DS [1 ]
DESSER, SS [1 ]
HONG, H [1 ]
机构
[1] UNIV TORONTO, DEPT ZOOL, TORONTO M5S 1A1, ONTARIO, CANADA
关键词
D O I
10.2307/3283481
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Six metabolic enzymes, glucose-6-phosphate dehydrogenase, glucosephosphate isomerase, isocitrate dehydrogenase, malate dehydrogenase, phosphoglucomutase, and purine nucleoside phosphorylase, from clonal isolates of 3 presumptive species of Trypanosoma (T. fallisi, T. ranarum, and T. rotatorium) from 3 anuran hosts (Bufo americanus, Rana clamitans, and Rana catesbeiana) were compared using starch-gel electrophoresis. Although bands were shared among the different zymodemes of isolates of the same host genus, low genetic polymorphism of the enzyme loci was observed with few apparent shared bands between samples isolated from frogs and toads. A distance value calculated between toad and frog trypanosome isolates suggests the likelihood of long-time separation of species. Cluster analysis based on overall similarity distinguished the trypanosomes of toads and frogs as separate taxa, suggesting that host specificity and observed morphological differences are consistent with heritable allozyme differences.
引用
收藏
页码:317 / 322
页数:6
相关论文
共 19 条
[1]  
[Anonymous], 1976, HDB ENZYME ELECTROPH
[2]  
AVISE JC, 1975, EVOLUTION, V29, P411, DOI 10.1111/j.1558-5646.1975.tb00831.x
[3]  
BARTA J R, 1984, Journal of Wildlife Diseases, V20, P180
[4]  
Brewer G. J, 1970, INTRO ISOZYME TECHNI
[5]   ULTRASTRUCTURE OF EPIMASTIGOTE STAGES OF TRYPANOSOMA-ROTATORIUM IN LEECH BATRACOBDELLA-PICTA [J].
DESSER, SS .
CANADIAN JOURNAL OF ZOOLOGY, 1976, 54 (10) :1712-1723
[6]  
DIAMOND L S, 1965, Wildl Dis, V44, P1
[7]   A COMPARISON OF THE ISOENZYMES OF TRYPANOSOMA-(DUTTONELLA)-VIVAX ISOLATES FROM EAST-AFRICA AND WEST-AFRICA [J].
FASOGBON, AI ;
KNOWLES, G ;
GARDINER, PR .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1990, 20 (03) :389-394
[8]   MISUSE AND MODIFICATION OF NEIS GENETIC-DISTANCE [J].
HILLIS, DM .
SYSTEMATIC ZOOLOGY, 1984, 33 (02) :238-240
[9]  
Jaffe C. L., 1984, Genes and antigens of parasites. A laboratory manual. Proceedings of a course held 14 November-17 December 1983, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., P47
[10]   A LIGHT AND ELECTRON-MICROSCOPIC STUDY OF TRYPANOSOMA-FALLISI N-SP IN TOADS (BUFO-AMERICANUS) FROM ALGONQUIAN PARK, ONTARIO [J].
MARTIN, DS ;
DESSER, SS .
JOURNAL OF PROTOZOOLOGY, 1990, 37 (03) :199-206