GENETIC-RECOMBINATION IN BROME MOSAIC-VIRUS - EFFECT OF SEQUENCE AND REPLICATION OF RNA ON ACCUMULATION OF RECOMBINANTS

被引:52
作者
NAGY, PD
BUJARSKI, JJ
机构
[1] NO ILLINOIS UNIV,CTR PLANT MOLEC BIOL,DE KALB,IL 60115
[2] NO ILLINOIS UNIV,DEPT BIOL SCI,DE KALB,IL 60115
关键词
D O I
10.1128/JVI.66.11.6824-6828.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In order to facilitate the isolation of recombinants in brome mosaic virus, a series of duplication mutants with alterations in the RNA3 3' noncoding region has been engineered. The distribution of crossovers, which was observed to be dependent on the parental RNA3 sequence, supported the role of RNA structure in recombination. However, a negative correlation between replication of the parental RNA3 constructs and the accumulation of recombinant progeny confirmed the role of selection.
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页码:6824 / 6828
页数:5
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