ASSOCIATION OF THE HALOBACTERIAL-7S RNA TO THE POLYSOME CORRELATES WITH EXPRESSION OF THE MEMBRANE-PROTEIN BACTERIOOPSIN

被引：44

作者：

GROPP, R ^{[1
]}

GROPP, F ^{[1
]}

BETLACH, MC ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 04期

关键词：

BACTERIORHODOPSIN; ARCHAEBACTERIA; SIGNAL-RECOGNITION PARTICLE; SIGNAL SEQUENCE;

D O I：

10.1073/pnas.89.4.1204

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. These two RNAs cosedimented predominantly with membrane-bound polysomes, and the quantity of 7S RNA bound to the ribosomes was directly correlated with the expression of bacterioopsin. Puromycin treatment released the 7S RNA from the polysomes, indicating that it is transiently associated with protein translation. We suggest that halobacteria contain a signal-recognition-like particle involved in translation of membrane-associated proteins.

引用

页码：1204 / 1208

页数：5

共 38 条

[1] THE HALO-OPSIN GENE .2. SEQUENCE, PRIMARY STRUCTURE OF HALORHODOPSIN AND COMPARISON WITH BACTERIORHODOPSIN [J].

BLANCK, A ;

OESTERHELT, D .

EMBO JOURNAL, 1987, 6 (01) :265-273

[2] PRIMARY STRUCTURE OF SENSORY RHODOPSIN-I, A PROKARYOTIC PHOTORECEPTOR [J].

BLANCK, A ;

OESTERHELT, D ;

FERRANDO, E ;

SCHEGK, ES ;

LOTTSPEICH, F .

EMBO JOURNAL, 1989, 8 (13) :3963-3971

[3] GENES FOR 7S RNAS CAN REPLACE THE GENE FOR 4.5S RNA IN GROWTH OF ESCHERICHIA-COLI [J].

BROWN, S .

JOURNAL OF BACTERIOLOGY, 1991, 173 (05) :1835-1837

[4] MUTATIONS IN THE GENE FOR EF-G REDUCE THE REQUIREMENT FOR 4.5S RNA IN THE GROWTH OF ESCHERICHIA-COLI [J].

BROWN, S .

CELL, 1987, 49 (06) :825-833

[5] BACTERIORHODOPSIN - PARTIAL SEQUENCE OF MESSENGER-RNA PROVIDES AMINO-ACID-SEQUENCE IN THE PRECURSOR REGION [J].

CHANG, SH ;

MAJUMDAR, A ;

DUNN, R ;

MAKABE, O ;

RAJBHANDARY, UL ;

KHORANA, HG ;

OHTSUKA, E ;

TANAKA, T ;

TANIYAMA, YO ;

IKEHARA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3398-3402

[6]

DELLWEG HG, 1980, FEBS LETT, V116, P303, DOI 10.1016/0014-5793(80)80668-5

[7] THE ROLE OF STRESS PROTEINS IN MEMBRANE BIOGENESIS [J].

DESHAIES, RJ ;

KOCH, BD ;

SCHEKMAN, R .

TRENDS IN BIOCHEMICAL SCIENCES, 1988, 13 (10) :384-388

[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[9] PROTEIN TRANSLOCATION ACROSS THE ENDOPLASMIC-RETICULUM .1. DETECTION IN THE MICROSOMAL MEMBRANE OF A RECEPTOR FOR THE SIGNAL RECOGNITION PARTICLE [J].

GILMORE, R ;

BLOBEL, G ;

WALTER, P .

JOURNAL OF CELL BIOLOGY, 1982, 95 (02) :463-469

[10] STRUCTURE OF THE ARCHAEBACTERIAL 7S RNA MOLECULE [J].

KAINE, BP .

MOLECULAR & GENERAL GENETICS, 1990, 221 (03) :315-321

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