SECRETION OF ALPHA-1-ANTITRYPSIN BY ALVEOLAR EPITHELIAL-CELLS

被引：67

作者：

VENEMBRE, P ^{[1
]}

BOUTTEN, A ^{[1
]}

SETA, N ^{[1
]}

DEHOUX, MS ^{[1
]}

CRESTANI, B ^{[1
]}

AUBIER, M ^{[1
]}

DURAND, G ^{[1
]}

机构：

[1] HOP BICHAT,INSERM,U408,F-75877 PARIS 18,FRANCE

来源：

FEBS LETTERS | 1994年 / 346卷 / 2-3期

关键词：

GLYCOSYLATION; A549 CELL LINE; TYPE-II PNEUMOCYTE; HUMAN; RAT;

D O I：

10.1016/0014-5793(94)80695-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the ability of alveolar epithelial cells (human A549 cell line and rat type-II pneumocytes) to produce al-antitrypsin (AAT). Northern blot analysis demonstrated the presence of an AAT-specific mRNA transcript in A549 cells. Unstimulated A549 cells secreted immunoreactive AAT at a rate of 0.51 +/- 0.04 ng/10(6) cells/h, with a modified glycosylation compared to serum AAT. AAT formed a complex with neutrophil elastase. Rat type-II pneumocytes secreted immunoreactive AAT. Our results suggest that alveolar epithelial cells could participate in antiprotease defense within the lung through local AAT production.

引用

页码：171 / 174

页数：4

共 26 条

[1] THE ASSESSMENT OF ALPHA-1-PROTEINASE INHIBITOR FORM AND FUNCTION IN LUNG LAVAGE FLUID FROM HEALTHY-SUBJECTS [J].