POTENTIAL ALLELOCHEMICALS FROM RUTA-GRAVEOLENS L AND THEIR ACTION ON RADISH SEEDS

被引:32
作者
ALIOTTA, G
CAFIERO, G
DEFEO, V
SACCHI, R
机构
[1] UNIV NAPLES FEDERICO II,CTR INTERDIPARTIMENTALE RIC ULTRASTRUTTURE BIOL,I-80139 NAPLES,ITALY
[2] UNIV NAPLES FEDERICO II,DIPARTIMENTO CHIM SOSTANZE NAT,I-80131 NAPLES,ITALY
[3] UNIV NAPLES FEDERICO II,IST CHIM AGR,I-80055 PORTICI,ITALY
关键词
ALEURONE; 5-METHOXYPSORALEN; 8-METHOXYPSORALEN; 4-HYDROXYCOUMARIN; COUMARINS; DORMANCY; LIGHT; RADISH; RAPHANUS SATIVUS; ROOT INHIBITION; RUTA GRAVEOLENS RUE; SEED COAT;
D O I
10.1007/BF02098388
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An aqueous extract of Ruta graveolens L. (250 g/liter) was tested for its allelopathic activity in vitro on radish germination and radicle growth in light and darkness. It caused a delay in the onset and a decrease in the rate of germination (40%) in the light. The photoinhibition of germination was accompanied by an inhibition of water uptake into the seed. Furthermore, the inhibition of radicle growth was slightly higher in the light than in darkness. Three potential allelochemicals, biologically active in the light, were isolated from the extract: 5-methoxypsoralen (5-MOP), 8-methoxypsoralen (8-MOP), and 4-hydroxy-coumarin at concentrations of 10(-4) M, 2 x 10(-4) M, and 0.4 x 10(-5) M respectively. At a concentration of 2 x 10(-4) M, 5-MOP was the most potent inhibitor, decreasing radish germination to 32% and radicle growth to 17% with respect to control. Microscopic observations of radish seeds treated with 5-MOP suggest that this substance changes the swelling of the seed coat and aleurone layer, which precedes radicle protrusion.
引用
收藏
页码:2761 / 2775
页数:15
相关论文
共 18 条
  • [1] Aliotta G., 1992, Giornale Botanico Italiano, V126, P631
  • [2] INHIBITION OF RADISH GERMINATION AND ROOT-GROWTH BY COUMARIN AND PHENYLPROPANOIDS
    ALIOTTA, G
    CAFIERO, G
    FIORENTINO, A
    STRUMIA, S
    [J]. JOURNAL OF CHEMICAL ECOLOGY, 1993, 19 (02) : 175 - 183
  • [3] BENEZRA C, 1985, PLANT CONTACT DERMAT
  • [4] BREWLEY JD, 1985, SEEDS PHYSL DEV GERM
  • [5] C-13 MAGNETIC-RESONANCE SPECTROSCOPY OF COUMARINS - C-13-PROTON LONG-RANGE COUPLINGS
    CHANG, CJ
    FLOSS, HG
    STECK, W
    [J]. JOURNAL OF ORGANIC CHEMISTRY, 1977, 42 (08) : 1337 - 1340
  • [6] Einhellig F. A., 1986, The science of allelopathy, P171
  • [7] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTRA .11. C-13 NMR CHEMICAL-SHIFTS AND CARBON-PROTON COUPLING-CONSTANTS OF SOME FUROCOUMARINS AND FUROCHROMONES
    ELGAMAL, MHA
    ELEWA, NH
    ELKHRISY, EAM
    DUDDECK, H
    [J]. PHYTOCHEMISTRY, 1979, 18 (01) : 139 - 143
  • [8] Lock-de-Ugaz O., 1988, INVESTIGACION FITOQU
  • [9] IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS
    LUFT, JH
    [J]. JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY, 1961, 9 (02): : 409 - &
  • [10] ADENOSINE PHOSPHATES IN GERMINATING RADISH (RAPHANUS-SATIVUS L) SEEDS
    MORELAND, DE
    HUSSEY, GG
    SHRINER, CR
    FARMER, FS
    [J]. PLANT PHYSIOLOGY, 1974, 54 (04) : 560 - 563