A NONENZYMATIC METHOD FOR LENS DECAPSULATION WHICH LEAVES THE EPITHELIAL-CELLS ATTACHED TO THE FIBERS

被引：4

作者：

DEWEY, J

BARTLING, C

RAE, JL

机构：

[1] MAYO CLIN & MAYO FDN,DEPT PHYSIOL & BIOPHYS,ROCHESTER,MN 55905

[2] MAYO CLIN & MAYO FDN,DEPT OPHTHALMOL,ROCHESTER,MN 55905

来源：

CURRENT EYE RESEARCH | 1995年 / 14卷 / 05期

关键词：

LENS; DECAPSULATION; DYE INJECTION; PATCH CLAMP; FLUORESCENCE;

D O I：

10.3109/02713689508999933

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

We have developed a method to mechanically decapsulate the lens yet leave the epithelial cells attached to the fibers. This method uses divalent ion chelaters to loosen the capsule-epithelial interactions and bumetanide to control cell swelling. Light microscopy, scanning electron microscopy, and in-vitro fluorescence microscopy demonstrate that the capsule is removed and the epithelial cells remain adherent to the fibers when these procedures are used. Ion channel activity and epithelial gap junction communication remain following decapsulation. This decapsulated lens preparation should prove useful for many kinds of lens studies.

引用

页码：357 / 362

页数：6

共 5 条

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