BETA-ENDORPHIN - CHARACTERISTICS OF BINDING-SITES IN THE RAT-BRAIN

被引：77

作者：

FERRARA, P

HOUGHTEN, R

LI, CH

机构：

[1] Hormone Research Laboratory University of California, San Francisco

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91847-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stereospecific binding of human β-endorphin to rat membrane preparations is described for the first time using [3H-Tyr27]-βh-endorphin as the ligand. The binding is time dependent and saturable with respect to βh-endorphin with an apparent dissociation constant of 0.3 nM. Sodium ion (100 mM) elevates this value to 2.5 nM but has no effect on the total number of binding sites present in the membrane preparation. The ability of certain β-endorphin analogs, opiate agonists as well as antagonists to inhibit the binding of βh-endorphin, is presented. © 1979.

引用

页码：786 / 792

页数：7

共 10 条

[1]

HOUGHTEN RA, 1978, INT J PEPT PROT RES, V12, P325

[2] SYNTHESIS AND ANALGESIC ACTIVITY OF HUMAN BETA-ENDORPHIN [J].

LI, CH ;

YAMASHIRO, D ;

TSENG, LF ;

LOH, HH .

JOURNAL OF MEDICINAL CHEMISTRY, 1977, 20 (03) :325-328

[3] ISOLATION AND STRUCTURE OF AN UNTRIAKONTAPEPTIDE WITH OPIATE ACTIVITY FROM CAMEL PITUITARY-GLANDS [J].

LI, CH ;

CHUNG, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (04) :1145-1148

[4]

LI CH, 1978, INT J PEPT PROT RES, V11, P154

[5]

LI CH, 1976, NATURE LONDON, V260, P623

[6] BETA-ENDORPHIN IS A POTENT ANALGESIC AGENT [J].

LOH, HH ;

TSENG, LF ;

WEI, E ;

LI, CH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (08) :2895-2898

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8]

PASTERNAK GW, 1975, MOL PHARMACOL, V11, P380

[9]

SWANN R, INT J PEPTIDE PROT R

[10]

TSENG LF, 1976, NATURE, V263, P239, DOI 10.1038/263239a0

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