MURINE SARCOMA VIRUS-ASSOCIATED PROTEIN-KINASE - INTERACTION WITH ACTIN AND MICROTUBULAR PROTEIN

被引：38

作者：

SEN, A

TODARO, GJ

机构：

[1] Laboratory of Viral Carcinogenesis National Cancer Institute Bethesda

来源：

CELL | 1979年 / 17卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0092-8674(79)90161-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A low molecular weight (LMW) protein phosphokinase enzyme that binds to actin has been isolated from murine sarcoma virions; this kinase activity is not present in nontransforming murine leukemia viruses. Sephadex G-75 gel filtration and affinity chromatography on actin-Sepharose conjugates allow a significant level of purification of this enzyme. The enzyme associates with microtubular proteins and inhibits the in vitro polymerization of microtubules. This study represents the first isolation of a sarcoma virus-associated protein that possesses the ability to interact directly with two major components of the cytoskeletal system. © 1979.

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页码：347 / 356

页数：10

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