STIMULATION AND PRIMING OF HUMAN NEUTROPHILS BY GRANULOCYTE COLONY-STIMULATING FACTOR AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR - QUALITATIVE AND QUANTITATIVE DIFFERENCES

Granulocyte colony-stimulating factor(G-CSF) and granulocyte-macrophage colony-stimulating factor(GM-CSF) increased neutrophil C3bi-receptor expression and adherence and rapidly (<10 min) primed neutrophils to enhance O2- release and membrane depolarization stimulated by chemotactic peptide. Direct triggering of O2- release in suspended neutrophils was also provoked by GM-CSF but not by G-CSF. GM-CSF-induced O2- release was inhibited by cyclic AMP agonists and cytochalasin B. The biological activity was greater in non-glycosylated GM-CSF than in glycosylated GM-CSF, whereas it was identical in glycosylated and non-glycosylated G-CSFs. Direct stimulation and priming by GM-CSF were consistently greater than those by G-CSF resulted in the effects of GM-CSF alone. These findings indicate that the effects of G-CSF and GM-CSF on neutrophil functions are qualitatively and quantitatively different from each other. © 1990.