CLONING AND SEQUENCING OF THE GILTHEAD SEABREAM (SPARUS-AURATA) GROWTH HORMONE-ENCODING CDNA

被引：42

作者：

FUNKENSTEIN, B

CHEN, TT

POWERS, DA

CAVARI, B

机构：

[1] STANFORD UNIV,HOPKINS MARINE STN,PACIFIC GROVE,CA 93950

[2] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

[3] UNIV MARYLAND,CTR MARINE BIOTECHNOL,BALTIMORE,MD 21202

来源：

GENE | 1991年 / 103卷 / 02期

关键词：

RECOMBINANT DNA; PITUITARY GLAND; RNA BLOT HYBRIDIZATION; NUCLEOTIDE AND AMINO ACID SEQUENCES; FISH; RAINBOW TROUT;

D O I：

10.1016/0378-1119(91)90280-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNA clones encoding gilthead seabream (gsb) (Sparus aurata) growth hormone (GH) have been isolated from a cDNA library prepared from seabream pituitary gland poly(A)+ RNA. The cDNA library was screened using red seabream and rainbow trout GH cDNAs. The complete nucleotide (nt) sequence of gsbGH has been determined. The cDNA sequence codes for a polypeptide of 204 amino acids (aa), including a putative signal peptide of 17 aa. The 5'- and 3'-untranslated regions of the message are 55 and 236 nt long, respectively. The predicted aa sequence of gsbGH revealed 97% homology with red seabream GH, 95% with tuna GH, 85% with yellowtail GH, and 65% with rainbow trout GH.

引用

页码：243 / 247

页数：5

共 32 条

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