EXPERIMENTAL STUDIES ON THE MECHANISM OF ACTION OF 4-HYDROXY-2,3-TRANS-NONENAL, A LIPID-PEROXIDATION PRODUCT DISPLAYING CHEMOTACTIC ACTIVITY TOWARD RAT NEUTROPHILS

被引：25

作者：

ROSSI, MA ^{[1
]}

CURZIO, M ^{[1
]}

DIMAURO, C ^{[1
]}

FIDALE, F ^{[1
]}

GARRAMONE, A ^{[1
]}

ESTERBAUER, H ^{[1
]}

TORRIELLI, M ^{[1
]}

DIANZANI, MU ^{[1
]}

机构：

[1] GRAZ UNIV,INST BIOCHEM,A-8010 GRAZ,AUSTRIA

来源：

CELL BIOCHEMISTRY AND FUNCTION | 1991年 / 9卷 / 03期

关键词：

INFLAMMATION; NEUTROPHILS; LIPID PEROXIDATION; 4-HYDROXYNONENAL; CHEMOTAXIS; PHOSPHOLIPASE-C;

D O I：

10.1002/cbf.290090304

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of 4-hydroxy-2,3-trans-nonenal (HNE) and nonanal on the activity of phosphoinositide-specific phospholipase C of rat neutrophils have been studied in parallel with their action on neutrophil oriented migration. Concentrations of HNE ranging from 10(-7) to 10(-5) M significantly stimulated the oriented migration of rat polymorphonuclear leukocytes. HNE stimulated both the basal and GTP gamma S-induced phospholipase C activity when used at concentrations between 10(-8) and 10(-6) M. Nonanal was devoid both of chemotactic activity and of any action on phospholipase C activity. The effect of GTP gamma S on the stimulation of phospholipase C induced by HNE was higher when the lowest dose of the aldehyde was used; the finding of an additive effect between 10(-8) M HNE and 2 x 10(-5) M GTP gamma S suggests that the two compounds may share a final common pathway of action. These results suggest that the chemotactic activity of HNE might be mediated, like that of other more well-known chemoattractants, by the stimulation of phosphoinositide-specific phospholipase C.

引用

页码：163 / 170

页数：8

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