The potential of lectins for analysis of food-grade galactomannans: double-diffusion lectin assay

被引：5

作者：

Patel, P. D. ^{[1
]}

Hawes, G. B. ^{[2
]}

机构：

[1] Leatherhead Food Res Assoc, Biopolymers Sect, Leatherhead KT22 7RY, Surrey, England

[2] Queensland Agr Coll, Gatton, Qld 4343, Australia

来源：

FOOD HYDROCOLLOIDS | 1988年 / 2卷 / 03期

关键词：

D O I：

10.1016/S0268-005X(88)80016-X

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The potential of D-galaetose-speeific Bandeiraea simplicifolia lectin (BS-lectin) and Artocarpus integrifolia lectin (Jacalin lectin) for specific detection of the galactomannans of guar and locust bean gum (LBG), from polysaccharide mixtures and foods, has been investigated. In a double-diffusion assay (in agarose gel) using the BS-lectin the lower limit of detection of guar and LBG was 100 and 1000 mu g/ml respectively. In a similar assay the Jacalin lectin specifically detected guar, but not LBG, at a concentration; >= 50 mu g/ml. In addition. the Jacalin lectin specifically detected guar down to similar to 50-100 mu g/ml in blends of polysaccharides containing either guar/LBG/xanthan or guar/LBG. The double-diffusion Jacalin lectin assay successfully detected guar in commercial food products containing guar (minestrone soup and horeseradish sauce) and correctly classified the food (seafood soup) as negative when guar was not present.

引用

页码：179 / 185

页数：7