ROUTINE MONITORING OF CRYPTOSPORIDIUM OOCYSTS IN WATER USING FLOW-CYTOMETRY

被引：70

作者：

VESEY, G ^{[1
]}

SLADE, JS ^{[1
]}

BYRNE, M ^{[1
]}

SHEPHERD, K ^{[1
]}

DENNIS, PJ ^{[1
]}

FRICKER, CR ^{[1
]}

机构：

[1] THAMES WATER UTIL LTD,SPENCER HOUSE LABS,MANOR FARM RD,READING RG2 0JN,ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 75卷 / 01期

关键词：

D O I：

10.1111/j.1365-2672.1993.tb03413.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A flow cytometric method for the routine analysis of environmental water samples for the presence of Cryptosporidium oocysts has been developed. It uses a Coulter Epics Elite flow cytometer to examine water samples and to separate oocysts from contaminating debris by cell sorting. The sorted particles are then rapidly screened by microscopy. The method has been evaluated and compared with direct epifluorescence microscopy on 325 river, reservoir and drinking water samples. The technique was found to be more sensitive, faster and easier to perform than conventional epifluorescent microscopy for the routine examination of water samples for Cryptosporidium.

引用

页码：87 / 90

页数：4

共 6 条

[1]

BADENOCH J, 1990, CRYPTOSPORIDIUM WATE

[2]

GILMOR RA, 1990, THESIS U STRATHCLYDE

[3]

Rose J.B., 1988, P205

[4] TAKING THE EYE STRAIN OUT OF ENVIRONMENTAL CRYPTOSPORIDIUM ANALYSIS [J].

VESEY, G ;

SLADE, JS ;

FRICKER, CR .

LETTERS IN APPLIED MICROBIOLOGY, 1991, 13 (02) :62-65

[5] A NEW METHOD FOR THE CONCENTRATION OF CRYPTOSPORIDIUM OOCYSTS FROM WATER [J].

VESEY, G ;

SLADE, JS ;

BYRNE, M ;

SHEPHERD, K ;

FRICKER, CR .

JOURNAL OF APPLIED BACTERIOLOGY, 1993, 75 (01) :82-86

[6]

VESEY G, 1990, WATER SCI TECHNOL, V24, P165

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