IN-VIVO MICRODIALYSIS OF CORTICOTROPIN-RELEASING FACTOR (CRF) - CALCIUM-DEPENDENCE OF DEPOLARIZATION-INDUCED NEUROSECRETION OF CRF

被引：14

作者：

GABR, RW

GLADFELTER, WE

BIRKLE, DL

AZZARO, AJ

机构：

[1] W VIRGINIA UNIV,ROBERT C BYRD HLTH SCI CTR,DEPT NEUROL,MORGANTOWN,WV 26506

[2] W VIRGINIA UNIV,ROBERT C BYRD HLTH SCI CTR,DEPT BEHAV MED PSYCHIAT,MORGANTOWN,WV 26506

[3] W VIRGINIA UNIV,ROBERT C BYRD HLTH SCI CTR,DEPT PHARMACOL TOXICOL,MORGANTOWN,WV 26506

[4] W VIRGINIA UNIV,ROBERT C BYRD HLTH SCI CTR,DEPT PHYSIOL,MORGANTOWN,WV 26506

来源：

NEUROSCIENCE LETTERS | 1994年 / 169卷 / 1-2期

关键词：

MICRODIALYSIS; MEDIAN EMINENCE; NEUROPEPTIDE; HYPOTHALAMUS; NEUROENDOCRINE; CRF;

D O I：

10.1016/0304-3940(94)90357-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Corticotropin releasing factor (CRF) is a large neuropeptide which functions as a major neurotransmitter in physiological stress responses. We have developed a microdialysis method for detecting CRF release from the median eminence of anesthetized rats. Depolarizing concentrations of KCl increased release of CRF into the perfusion media; this effect was inhibited by 100 muM verapamil. Our characterization of the physiologic conditions of KCl-induced release of neuronal CRF using the microdialysis technique provides evidence that the CRF release system is Ca2+-dependent and maintains its integrity over many hours in anesthetized rats.

引用

页码：63 / 67

页数：5

共 28 条

[1] INFLUENCE OF THE CENTRAL NUCLEUS OF THE AMYGDALA ON THE CONTENT OF CORTICOTROPIN-RELEASING FACTOR IN THE MEDIAN-EMINENCE [J].