SEQUENCING PEPTIDES WITHOUT SCANNING THE REFLECTRON - POST-SOURCE DECAY WITH A CURVED-FIELD REFLECTRON TIME-OF-FLIGHT MASS-SPECTROMETER

被引:41
作者
CORDERO, MM
CORNISH, TJ
COTTER, RJ
LYS, IA
机构
[1] JOHNS HOPKINS UNIV,SCH MED,MIDDLE ATLANTIC MASS SPECTROMETRY LAB,BALTIMORE,MD 21205
[2] ILYS SOFTWARE,PITTSBURGH,PA 15232
关键词
D O I
10.1002/rcm.1290091407
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A time-of-flight mass spectrometer has been developed using a reflectron in which the voltages placed on the lens elements correspond to a function describing the are of a circle. This curved-field reflectron enables acquisition of focused product-ion mass spectra at a single reflectron voltage, In this paper, we present results from this instrument for the amino acid sequencing of several peptides and describe the method of mass calibration.
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页码:1356 / 1361
页数:6
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