CLONING OF THE RAT ERBB3 CDNA AND CHARACTERIZATION OF THE RECOMBINANT PROTEIN

被引：31

作者：

HELLYER, NJ ^{[1
]}

KIM, HH ^{[1
]}

GREAVES, CH ^{[1
]}

SIERKE, SL ^{[1
]}

KOLAND, JG ^{[1
]}

机构：

[1] UNIV IOWA, COLL MED, DEPT PHARMACOL, IOWA CITY, IA 52242 USA

来源：

GENE | 1995年 / 165卷 / 02期

关键词：

HER3; PROTEIN TYROSINE KINASE; SRC HOMOLOGY DOMAIN; HEREGULIN; ONCOGENE;

D O I：

10.1016/0378-1119(95)00436-A

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Three cDNA fragments that encoded all but the extreme N terminus of the rat ErbB3 protein were cloned by low-stringency screening of a rat liver cDNA library with a human ERBB3 probe. The remaining 5'-end of the cDNA was generated by a reverse transcription-polymerase chain reaction method, and a single full-length rat ErbB3 cDNA was assembled. A comparison of the deduced amino acid (aa) sequences of human and rat ErbB3 was made, and the effects of certain aa substitutions in the putative protein tyrosine kinase domain were considered. The rat ErbB3 cDNA was subsequently expressed in cultured NIH-3T3 mouse fibroblasts, in which a high level of approx. 180-kDa recombinant ErbB3 (re-ErbB3) was generated. The rat re-ErbB3 produced in transfected fibroblasts was responsive to the polypeptide, heregulin, a known ligand for ErbB3. Challenge of transfected fibroblasts with heregulin stimulated the phosphorylation of rat re-ErbB3 on Tyr residues and promoted its association with the p85 subunit of phosphatidylinositol 3-kinase. Together, these results indicate that a fully functional rat ErbB3 cDNA has been isolated, and that fibroblast cells expressing this cDNA will be suitable for investigations of the signal transduction mechanism of ErbB3.

引用

页码：279 / 284

页数：6

共 29 条

[1]

Carraway, Sliwkowski, Akita, Platko, Guy, Nuijens, Diamonti, Vandlen, Cantley, Cerione, The erbB3 gene product is a receptor for heregulin, J. Biol. Chem., 269, pp. 14303-14306, (1994)

[2]

Cohen, Ren, Baltimore, Modular binding domains in signal transduction proteins, Cell, 80, pp. 237-248, (1995)

[3]

Coker, Staros, Guyer, A kinase-negative epidermal growth factor receptor that retains the capacity to stimulate DNA synthesis, Proc. Natl. Acad. Sci. USA, 91, pp. 6967-6971, (1994)

[4]

Downward, Yarden, Mayes, Scrace, Totty, Stockwell, Ullrich, Schlessinger, Waterfield, Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences, Nature (London), 307, pp. 521-527, (1984)

[5]

Dumas, Edwards, Delort, Mallet, Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification, Nucleic Acids Res., 19, pp. 5227-5232, (1991)

[6]

Fedi, Pierce, Di, Kraus, Efficient coupling with phosphatidylinositol 3-kinase, but not phospholipase Cγ or GTPase-activating protein, distinguishes ErbB-3 signaling from that of other ErbB/EGFR family members, Mol. Cell. Biol., 14, pp. 492-500, (1994)

[7]

Gill, Bertics, Santon, Epidermal growth factor and its receptor, Mol. Cell. Endo., 51, pp. 169-186, (1987)

[8]

Guy, Platko, Cantley, Cerione, Carraway, Insect cell-expressed p180<sup>erbB3</sup> possesses an impaired tyrosine kinase activity, Proc. Natl. Acad. Sci. USA, 91, pp. 8132-8136, (1994)

[9]

Hanks, Quinn, Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members, Meth. Enzymol., 200, pp. 38-62, (1991)

[10]

Holmes, Sliwkowski, Akita, Henzel, Lee, Park, Yansura, Abadi, Raab, Lewis, Shepard, Kuang, Wood, Goeddel, Vandlen, Identification of heregulin, a specific activator of p<sup>185erbB2</sup>, Science, 256, pp. 1205-1210, (1992)

← 1 2 3 →