THE USE OF DIVALENT-CATION CHELATING AGETS (EDTA EGTA) TO REDUCE NONSPECIFIC SERUM-PROTEIN INTERACTION IN ENZYME-IMMUNOASSAY

被引：33

作者：

NIELSEN, K ^{[1
]}

KELLY, L ^{[1
]}

GALL, D ^{[1
]}

SMITH, P ^{[1
]}

BOSSE, J ^{[1
]}

NICOLETTI, P ^{[1
]}

KELLY, W ^{[1
]}

机构：

[1] UNIV FLORIDA, COLL VET MED, DEPT INFECT DIS, GAINESVILLE, FL 32611 USA

来源：

VETERINARY RESEARCH COMMUNICATIONS | 1994年 / 18卷 / 06期

关键词：

BRUCELLA-ABORTUS; CATTLE; CHELATING AGENTS; EDTA; EGTA; ELISA; IMMUNOASSAY; SPECIFICITY;

D O I：

10.1007/BF01839419

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An indirect enzyme immunoassay (ELISA) for detection of bovine antibody to Brucella abortus was modified by the addition of divalent chelating agents to the serum diluent. This addition resulted in an increase in specificity from 96.0% in the regular assay to 99.4% in the modified procedure. Of the 15 715 sera initially tested by the indirect ELISA, 691 that had given positive reactions were selected for retesting in the indirect ELISA with EDTA/EGTA added. The buffered plate antigen test (BPAT) correctly identified 98.6% of the samples as negative. The addition of chelating agents did not alter the sensitivity of the indirect ELISA, which correctly classified 609 sera from animals from which B. abortus had been isolated as positive. The sensitivity of the BPAT was 97.8%.

引用

页码：433 / 437

页数：5

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