SEXUAL CROSSES OF THE HOMOTHALLIC FUNGUS GAEUANNOMYCES-GRAMINIS VAR TRITICI BASED ON USE OF AN AUXOTROPH OBTAINED BY TRANSFORMATION

被引：19

作者：

PILGERAM, AL ^{[1
]}

HENSON, JM ^{[1
]}

机构：

[1] MONTANA STATE UNIV,DEPT MICROBIOL,BOZEMAN,MT 59717

来源：

EXPERIMENTAL MYCOLOGY | 1992年 / 16卷 / 01期

基金：

美国国家科学基金会;

关键词：

GAEUMANNOMYCES; TAKE-ALL; CROSS; NICOTINIC ACID; AUXOTROPH; TRANSFORMATION; FUNGUS;

D O I：

10.1016/0147-5975(92)90039-T

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transformation ofGaeumannomyces graminis, a homothallic ascomycete, to benomyl resistance (BenR) or phleomycin resistance (PhleoR) enabled us to isolate crossed perithecia and analyze phenotypic and genotypic segregation of the selectable markers in ascospore progeny. One BenR transformant required nicotinic acid for growth and the relationship between the auxotrophy and BenR was examined. Normal distributions of both benomyl resistance and phleomycin resistance were observed in ascospore progeny from crosses between the auxotroph and a wild-type strain or between the auxotroph and a PhleoR transformant. Benomyl resistance always segregated with nicotinic acid auxotrophy in the progeny, indicating that the two genes encoding these traits were closely linked. A simple method for faster production ofG. graminis perithecia is also described. © 1992 Academic Press, Inc.

引用

页码：35 / 43

页数：9

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