EXPERIMENTAL INFECTIONS AND NATURAL OUTBREAKS OF EPERYTHROZOONOSIS IN PIGS IDENTIFIED BY PCR-DNA HYBRIDIZATIONS

被引:5
作者
OBERST, RD
GWALTNEY, SM
HAYS, MP
MORGAN, S
STAIR, EL
机构
[1] Department of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan
[2] Boren Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Oklahoma State University, Stillwater
[3] Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater
关键词
D O I
10.1177/104063879300500308
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Eperythrozoon-specific DNA amplification reactions and subsequent hybridizations with an eperythrozoon DNA probe (KSU-2) were used in experimental infection studies to identify Eperythrozoon suis DNA in the blood of splenectomized and nonsplenectomized pigs. The results indicate that E. suis DNA is present in nonsplenectomized pigs at levels that can be amplified by polymerase chain reaction (PCR) and identified in DNA hybridizations within 24 hours after infection. The ability of the E. suis PCR/hybridization assay to detect eperythrozoonosis was further demonstrated in blood samples collected from pigs in 2 separate natural outbreaks in Oklahoma. Results from these initial samplings indicate that pigs infected with E. suis from geographically distinct locations can be identified using the eperythrozoon-specific PCR/hybridization assay, which offers many advantages over conventional laboratory procedures for diagnosing eperythrozoonosis in pigs.
引用
收藏
页码:351 / 358
页数:8
相关论文
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