PHYSICAL MAP OF THE LINEAR CHROMOSOME OF STREPTOMYCES-GRISEUS

被引：77

作者：

LEZHAVA, A ^{[1
]}

MIZUKAMI, T ^{[1
]}

KAJITANI, T ^{[1
]}

KAMEOKA, D ^{[1
]}

REDENBACH, M ^{[1
]}

SHINKAWA, H ^{[1
]}

NIMI, O ^{[1
]}

KINASHI, H ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIGASHIHIROSHIMA 724,JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 22期

关键词：

D O I：

10.1128/jb.177.22.6492-6498.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The chromosomal DNA of Streptomyces griseus 2247 (a derivative of strain IFO3237) was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE), Digestion with AseI and DraI gave 15 and 9 fragments, respectively, the total sizes of which were 7.8 Mb. All the AseI and DraI fragments were aligned on a linear chromosome map by using linking plasmids and cosmids, PFGE analysis of the intact chromosome also showed a linear DNA band of about 8 Mb, Detailed physical maps of both terminal regions were constructed; they revealed the presence of a 24-kb terminal inverted repeat on each end. PFGE analysis with and without proteinase K treatment suggested that each end of the chromosome carries a protein molecule.

引用

页码：6492 / 6498

页数：7

共 39 条

[1] PRESENCE OF ONE LINEAR AND ONE CIRCULAR CHROMOSOME IN THE AGROBACTERIUM-TUMEFACIENS C58 GENOME [J].

ALLARDETSERVENT, A ;

MICHAUXCHARACHON, S ;

JUMASBILAK, E ;

KARAYAN, L ;

RAMUZ, M .

JOURNAL OF BACTERIOLOGY, 1993, 175 (24) :7869-7874

[2]

BERDY J, 1980, PROCESS BIOCHEM, V15, P28

[3]

Chater K. F., 1993, P83

[4] GENETICS OF DIFFERENTIATION IN STREPTOMYCES [J].

CHATER, KF .

ANNUAL REVIEW OF MICROBIOLOGY, 1993, 47 :685-713

[5] THE CONJUGATIVE PLASMID SLP2 OF STREPTOMYCES-LIVIDANS IS A 50 KB LINEAR MOLECULE [J].

CHEN, CW ;

YU, TW ;

LIN, YS ;

KIESER, HM ;

HOPWOOD, DA .

MOLECULAR MICROBIOLOGY, 1993, 7 (06) :925-932

[6]

CHEN CW, ACTINOMYCETOLOGICA, V8, P103

[7] SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].

CHU, G ;

VOLLRATH, D ;

DAVIS, RW .

SCIENCE, 1986, 234 (4783) :1582-1585

[8] LARGE GENOMIC REARRANGEMENTS OF THE UNSTABLE REGION IN STREPTOMYCES-AMBOFACIENS ARE ASSOCIATED WITH MAJOR CHANGES IN GLOBAL GENE-EXPRESSION [J].

DARY, A ;

KAISER, P ;

BOURGET, N ;

THOMPSON, CJ ;

SIMONET, JM ;

DECARIS, B .

MOLECULAR MICROBIOLOGY, 1993, 10 (04) :759-769

[9] PHYSICAL MAP OF THE LINEAR CHROMOSOME OF THE BACTERIUM BORRELIA-BURGDORFERI-212, A CAUSATIVE AGENT OF LYME-DISEASE, AND LOCALIZATION OF RIBOSOMAL-RNA GENES [J].

DAVIDSON, BE ;

MACDOUGALL, J ;

GIRONS, IS .

JOURNAL OF BACTERIOLOGY, 1992, 174 (11) :3766-3774

[10] GENETIC INSTABILITY AND DNA AMPLIFICATION IN STREPTOMYCES-LIVIDANS 66 [J].

DYSON, P ;

SCHREMPF, H .

JOURNAL OF BACTERIOLOGY, 1987, 169 (10) :4796-4803

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