REPRODUCIBILITY OF THE SHORT INSULIN TOLERANCE-TEST

被引：56

作者：

HIRST, S ^{[1
]}

PHILLIPS, DIW ^{[1
]}

VINES, SK ^{[1
]}

CLARK, PM ^{[1
]}

HALES, CN ^{[1
]}

机构：

[1] UNIV SOUTHAMPTON,SOUTHAMPTON GEN HOSP,MRC,ENVIRONM EPIDEMIOL UNIT,METAB PROGRAMMING GRP,SOUTHAMPTON S09 4XY,ENGLAND

来源：

DIABETIC MEDICINE | 1993年 / 10卷 / 09期

关键词：

INSULIN RESISTANCE; REPRODUCIBILITY; INSULIN TOLERANCE TEST;

D O I：

10.1111/j.1464-5491.1993.tb00176.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The short insulin tolerance test is a simple method of estimating insulin resistance by measuring the rate of fall of blood glucose following the intravenous administration of insulin. To determine its reproducibility, 18 healthy subjects underwent duplicate insulin tolerance tests separated by at least 1 week. Intravenous insulin (0.05 units kg-1) was administered into an antecubital vein and arterialized venous samples were obtained from a retrogradely cannulated vein on the dorsum of the hand on the same side. The test was terminated with an intravenous glucose injection 15 min after the administration of insulin. The mean whole blood glucose concentration fell from 4.6 mmol l-1 to 2.8 mmol l-1 while plasma insulin concentrations rose to supraphysiological leveLs and decLined exponentially. The glucose disappearance rate (K(itt)) calculated from the slope of the fall in log transformed blood glucose between 3 and 15 min after insulin injection ranged from 2.1 to 6.5 (mean 4.4) % min-1 during the first visit and 1.7 to 7.4 (mean 4.4) % min-1 during the second. The ratio of the within-subject to between-subject variance of the test was 0.24, the within-subject coefficient of variation was 13 % and the between-subject coefficient of variation 26 %. The short insulin tolerance test is reproducible and could be used to measure insulin resistance in large-scale epidemiological studies.

引用

页码：839 / 842

页数：4

共 11 条

[1]

DeFronzo RA, Tobin JD, Andres R., The glucose clamp technique: a method of quantifying insulin secretion and resistance, Am J Physiol, 237, (1979)

[2]

Bonora E., Moghetti P., Zancanaro C., Cigolini M., Querena M., Cacciatori V., Et al., Estimates of in vivo insulin action in man: comparison of insulin tolerance tests with euglycaemic and hyperglycaemic glucose clamp studies, J Clin Endocrinol Metab, 68, pp. 374-378, (1989)

[3]

Akinmokun A., Selby PL, Ramaiya K., Alberti KGMM, The short insulin tolerance test for determination of insulin sensitivity: A comparison with the euglycaemic clamp, Diabetic Med, 9, pp. 432-437, (1992)

[4]

Dornhorst A., Edwards SGM, Nicholls JSD, Anyaoku V., McLaren D., Heslop KE, Et al., A defect in insulin release in women at risk of future non‐insulin‐dependent diabetes, Clin Sci, 81, pp. 195-199, (1991)

[5]

Robinson S., Chan S-P, Spacey S., Anyaoku V., Johnston DG, Franks S., Postprandial thermogenesis is reduced in polycystic ovary syndrome and is associated with increased insulin resistance, Clin Endocrinol (Oxf), 36, pp. 537-543, (1992)

[6]

Sobey WJ, Beer SF, Carrington CA, Clark PMS, Frank BH, Gray IP, Et al., Sensitive and specific two‐site immunoradiometric assay for human insulin, and proinsulin, 65–66 split and 32–33 split proinsulins, Biochem J, 260, pp. 535-541, (1989)

[7]

Ferrari P., Alleman Y., Shaw S., Riesen W., Weidmann P., Reproducibility of insulin sensitivity measured by the minimal model method, Diabetologia, 34, pp. 527-530, (1991)

[8]

Nagi DK, Yudkin JS, The reproducibility of the insulin sensitivity test (IST) without pancreatic suppression (modified Harano technique) as an index of insulin resistance in non‐insulin‐dependent diabetic subjects, Diabetic Med, (1990)

[9]

Pollare T., Lithell H., Berne C., A comparison of the effects of hydrochlorothiazide and captopril on glucose and lipid metabolism in patients with hypertension, N Engl J Med, 321, pp. 868-873, (1989)

[10]

Yudkin JS, Alberti KGMM, McLarty DG, Swai ABM, Impaired glucose tolerance. Is it a risk factor for diabetes or a diagnostic ratbag?, Br Med J, 301, pp. 397-402, (1991)

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