VIP17/MAL, a proteolipid in apical transport vesicles

被引：101

作者：

Zacchetti, D ^{[1
]}

Peranen, J ^{[1
]}

Murata, M ^{[1
]}

Fiedler, K ^{[1
]}

Simons, K ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,CELL BIOL PROGRAMME,D-69012 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1995年 / 377卷 / 03期

关键词：

Madin-Darby canine kidney cell; proteolipid; apical transport; myelin biogenesis; detergent-insoluble complex; vesicular trafficking;

D O I：

10.1016/0014-5793(95)01396-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

VIP17 is a proteolipid enriched in the CHAPS-insoluble complexes from MDCK cells, and a candidate component of the molecular machinery responsible for the sorting and targeting of proteins to the apical surface, Cloning and sequencing of the cDNA encoding the protein revealed that it is the canine homolog of the human and rat MAL proteins. Analysis by immunofluorescence microscopy of epitope-tagged VIP17/MAL expressed transiently in BHK cells and stably in MDCK cells revealed a perinuclear, vesicular, and plasmalemmal staining. In MDCK cells the distribution,vas mainly in vesicular structures in the apical cytoplasm. These and other results suggest that VIP17/MAL is an important component in vesicular trafficking cycling between the Golgi complex and the apical plasma membrane.

引用

页码：465 / 469

页数：5

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