INHIBITORY EFFECT OF NIVALENOL A TOXIC METABOLITE OF FUSARIUM NIVALE ON GROWTH CYCLE AND BIOPOLYMER SYNTHESIS OF HELA CELLS

被引:41
作者
OHTSUBO, K
YAMADA, MA
SAITO, M
机构
[1] Department of Pathology, Faculty of Medicine, the University of Tokyo
[2] Department of Pathology, National Institute of Health
[3] Department of Carcinogenesis and Cancer Susceptibility, Institute of Medical Science, the University of Tokyo
来源
JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY | 1968年 / 21卷 / 03期
关键词
D O I
10.7883/yoken1952.21.185
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Nivalenol, a toxic product isolated from the rice grains infected with Fusarium nivale, was shown to inhibit the multiplication of HeLa cells completely at a concentration of 0.5μg/ml or higher. At the concentration of 5μg/ml, protein and DNA syntheses were almost entirely suppressed, whereas little or no inhibition took place in RNA synthesis. The inhibitory effects on both DNA and protein syntheses were similar with respect to the rapidity and severity. Cell cycle analysis revealed that, in addition to direct effect on S phase, the toxin affected the entry of G1 cells into S phase and that of G2 cells into mitosis. These results are discussed with special reference to the similarity of the action to that of well-known inhibitors for protein synthesis. © 1968, National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee. All rights reserved.
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页码:185 / &
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