QUANTITATION BY RADIOIMMUNOASSAY OF PAF IN HUMAN SALIVA

被引:9
作者
COONEY, SJ
SMAL, MA
BALDO, BA
机构
[1] ROYAL N SHORE HOSP,KOLLING INST MED RES,ST LEONARDS,NSW 2065,AUSTRALIA
[2] UNIV SYDNEY,DEPT MED,SYDNEY,NSW 2006,AUSTRALIA
关键词
D O I
10.1007/BF02536518
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Approximately 75% of the PAF present in saliva is recovered on extraction of whole saliva (0.8 vol) with chloroform/methanol/water (2:2:1, v/v/v). PAF levels, determined by our recently developed radioimmunoassay, in saliva extracts ranged from 0.5-21 ng/mL with 59% between 2-6 ng/mL. These figures, for apparently healthy subjects, are higher than previously reported levels obtained by platelet assays. The validity of our radioimmunoassay results was checked by isolating and quantitating the PAF fraction from whole saliva. In addition, when we examined our saliva samples by platelet aggregation, low levels of PAF, comparable with the values found in the literature, were detected. Investigations revealed the presence of a substance(s) which inhibited PAF-induced platelet aggregation but which did not affect the radioimmunoassay.
引用
收藏
页码:1140 / 1143
页数:4
相关论文
共 19 条
[1]   A SPECIFIC, SENSITIVE AND HIGH-CAPACITY IMMUNOASSAY FOR PAF [J].
BALDO, BA ;
SMAL, MA ;
MCCASKILL, AC .
LIPIDS, 1991, 26 (12) :1136-1139
[2]   PLATELET-ACTIVATING FACTOR AS A MEDIATOR OF ALLERGIC DISEASE [J].
BARNES, PJ ;
CHUNG, KF ;
PAGE, CP .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1988, 81 (05) :919-934
[3]   PLATELET-ACTIVATING FACTOR AND ASTHMA [J].
BARNES, PJ .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1988, 81 (01) :152-160
[4]   RELEASE OF PLATELET-ACTIVATING FACTOR (PAF-ACETHER) AND 2-LYSO PAF-ACETHER FROM 3 CELL-TYPES [J].
BENVENISTE, J ;
ROUBIN, R ;
CHIGNARD, M ;
JOUVINMARCHE, E ;
LECOUEDIC, JP .
AGENTS AND ACTIONS, 1982, 12 (5-6) :711-713
[5]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[6]   TRANSIENT ACTIVATION OF THE ACETYLTRANSFERASE NECESSARY FOR PAF-ACETHER BIOSYNTHESIS IN THROMBIN-ACTIVATED PLATELETS [J].
COEFFIER, E ;
NINIO, E ;
LECOUEDIC, JP ;
CHIGNARD, M .
BRITISH JOURNAL OF HAEMATOLOGY, 1986, 62 (04) :641-651
[7]  
COX CP, 1981, J IMMUNOL, V127, P46
[8]  
HANAHAN DJ, 1980, J BIOL CHEM, V255, P5514
[9]   PLATELET-ACTIVATING FACTOR ISOLATION, IDENTIFICATION, AND ASSAY [J].
HANAHAN, DJ ;
WEINTRAUB, ST .
METHODS OF BIOCHEMICAL ANALYSIS, 1985, 31 :195-219
[10]   PLATELET-ACTIVATING-FACTOR - CHEMICAL AND BIOCHEMICAL CHARACTERISTICS [J].
HANAHAN, DJ ;
KUMAR, R .
PROGRESS IN LIPID RESEARCH, 1987, 26 (01) :1-28