INS(1,3,4,5)P4 PROMOTES SUSTAINED ACTIVATION OF THE CA2+-DEPENDENT CL- CURRENT IN ISOLATED MOUSE LACRIMAL CELLS

被引：41

作者：

SMITH, PM

机构：

[1] Department of Physiology, University of Liverpool

来源：

BIOCHEMICAL JOURNAL | 1992年 / 283卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj2830027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Infusion of 50-mu-M-Ins(1,3,4,5)P4 in addition to 500-mu-M-Ins(1,4,5)P3 into mouse lacrimal cells via a patch-clamp pipette promoted sustained activation of the Ca2+-dependent Cl- current, which could not be achieved with 500-mu-M-Ins(1,4,5)P3 alone. It has been proposed that Ins(1,3,4,5)P4 facilitates Ca2+ influx in the presence of Ins(1,4,5)P3 [Morris, Gallacher, Irvine & Petersen (1987) Nature (London) 330, 653-655], but a subsequent study in mouse lacrimal cells [Bird, Rossier, Hughes, Shears, Armstrong & Putney (1991) Nature (London) 352, 162-165] showed that a high concentration of Ins(1,4,5)P3 could mobilize both intra- and extra-cellular Ca2+ in the absence of Ins(1,3,4,5)P4. My data confirm these findings, but also show that Ins(1,3,4,5)P4 can stimulate additional Ca2+ influx even when the Ins(1,4,5)P3-dependent intracellular Ca2+ pools have been depleted.

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页码：27 / 30

页数：4

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