DIHYDROPYRIMIDINE AMIDOHYDROLASE IS A ZINC METALLOENZYME

被引：15

作者：

BROOKS, KP ^{[1
]}

KIM, BD ^{[1
]}

SANDER, EG ^{[1
]}

机构：

[1] W VIRGINIA UNIV,SCH MED,DEPT BIOCHEM,MORGANTOWN,WV 26506

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 570卷 / 01期

关键词：

Dihydropyrimidine amidohydrolase; Zinc metalloenzyme;

D O I：

10.1016/0005-2744(79)90216-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine liver dihydropyrimidine amidohydrolase (EC 3.5.2.2) has been subjected to atomic absorption analysis. Three different preparations of homogeneous enzyme indicated that the enzyme contains 4.3 ± 0.3 g atoms of Zn2+ per mol of enzyme or 1.1 g atoms of Zn2+ per subunit. No Co2+, Mn2+, Mg2+ or Cd2+ was detected. Exhaustive dialysis against either o-phenanthroline or EDTA did not reduce enzyme activity; however, prolonged incubation with dipicolinic acid resulted in inactivation which can be reversed by either Zn2+ or Co2+ but not Mg2+. © 1979.

引用

页码：213 / 214

页数：2

共 8 条

[1]

DUDLEY KH, 1978, DRUG METAB DISPOS, V6, P133

[2]

DUDLEY KH, 1974, DRUG METAB DISPOS, V2, P103

[3]

KIM BD, 1976, J BIOL CHEM, V251, P6909

[4]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[5]

MAGUIRE JH, 1978, DRUG METAB DISPOS, V6, P601

[6]

MAGUIRE JH, 1978, DRUG METAB DISPOS, V6, P140

[7]

SANDER EG, 1965, J BIOL CHEM, V240, P3628

[8] PURIFICATION AND PROPERTIES OF DIHYDROOROTASE, A ZINC-CONTAINING METALLOENZYME IN CLOSTRIDIUM-OROTICUM [J].

TAYLOR, WH ;

TAYLOR, ML ;

BALCH, WE ;

GILCHRIST, PS .

JOURNAL OF BACTERIOLOGY, 1976, 127 (02) :863-873

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