LOCALIZATION OF CARBONIC-ANHYDRASE IN THE GILLS OF CARCINUS-MAENAS

被引：13

作者：

BOTTCHER, K

SIEBERS, D

BECKER, W

机构：

[1] UNIV HAMBURG,INST ZOOL,W-2000 HAMBURG 13,GERMANY

[2] UNIV HAMBURG,MUSEUM,W-2000 HAMBURG 13,GERMANY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 96卷 / 02期

关键词：

D O I：

10.1016/0305-0491(90)90370-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Gill hemogenates of Carcinus maenas were centrifuged at 100,000 g. Pellets and supernatants were analyzed for carbonic anhydrase (CA) activity. Approximately 70% of total CA activity occurred in a bound form, the rest being cytosolic. 2. 2. Repeated hemogenization and sonication prior to recentrifugation did not result in additional liberation of CA from the bound state. 3. 3. Centrifugation of a 7500 g pellet in a sucrose density gradiant provided evidence that the bound form of CA is present in plasma membranes. © 1990.

引用

页码：243 / 246

页数：4

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