EVIDENCE FOR GLUCOCORTICOID TRANSPORT INTO ATT-20 CELLS - SELECTIVE EFFECT OF A SULFHYDRYL INHIBITOR ON UPTAKE

被引:15
作者
HARRISON, RW [1 ]
YEAKLEY, J [1 ]
机构
[1] VANDERBILT UNIV,DEPT MED,NASHVILLE,TN 37232
关键词
mouse; pituitary cells; plasma membrane; steroid uptake; sulfhydryl groups;
D O I
10.1016/0303-7207(79)90066-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The purpose of these experiments was to determine if membrane-mediated glucocorticoid uptake by the AtT-20 cell was sensitive to the sulfhydryl group inhibitor, p-chloromercuri-phenylsulfonate (PCMPS). This agent was chosen because of its reported limited entry into the cell interior. Our experiments showed that cells could be incubated with 1 mM PCMPS for 1 h before intracellular concentrations of the inhibitor were sufficient to affect the intracellular cytosol receptor. In contrast less than 15 min of treatment inhibited intact cell steroid uptake. The inhibition was completely reversed by dithiothreitol. These studies show inhibition of glucocorticoid uptake under conditions which do not affect the intracellular receptor and infer that the AtT-20 cell membrane-mediated uptake mechanism for glucocorticoids contains sensitive sulfhydryl groups. © 1979.
引用
收藏
页码:13 / 18
页数:6
相关论文
共 12 条
[1]  
Baulieu E E, 1975, Vitam Horm, V33, P649
[2]   DISTINCTIVE ALTERATIONS OF NUCLEOSIDE, SUGAR AND AMINO-ACID UPTAKE BY SULFHYDRYL REAGENTS IN CULTURED MOUSE CELLS [J].
HARE, JD .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 170 (02) :347-352
[3]   EVIDENCE FOR GLUCOCORTICOID TRANSPORT THROUGH TARGET-CELL MEMBRANE [J].
HARRISON, RW ;
FAIRFIELD, S ;
ORTH, DN .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 61 (04) :1262-1267
[4]   EVIDENCE FOR GLUCOCORTICOID TRANSPORT INTO ATT-20-D-1 CELLS [J].
HARRISON, RW ;
FAIRFIELD, S ;
ORTH, DN .
BIOCHEMISTRY, 1975, 14 (06) :1304-1307
[5]   EFFECT OF CELL-MEMBRANE ALTERATION ON GLUCOCORTICOID UPTAKE BY ATT-20-D-1 TARGET-CELL [J].
HARRISON, RW ;
FAIRFIELD, S ;
ORTH, DN .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 466 (02) :357-365
[6]  
HARRISON RW, BIOCH BIOPHYS ACTA
[7]   STUDIES ON ESTROGEN ENTRY INTO UTERINE CELLS AND ON ESTRADIOL-RECEPTOR COMPLEX ATTACHMENT TO NUCLEUS - ENTRY OF ESTROGEN INTO UTERINE CELLS A PROTEIN-MEDIATED PROCESS [J].
MILGROM, E ;
ATGER, M ;
BAULIEU, EE .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 320 (02) :267-283
[8]   TRANSPORT OF NUCLEOSIDES, NUCLEIC-ACID BASES, CHOLINE AND GLUCOSE BY ANIMAL-CELLS IN CULTURE [J].
PLAGEMANN, PGW ;
RICHEY, DP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 344 (3-4) :263-305
[9]   EFFECTS OF SULFHYDRYL INHIBITORS UPON TRANSPORT OF FOLATE COMPOUNDS INTO L1210 CELLS [J].
RADER, JI ;
NIETHAMMER, D ;
HUENNEKENS, FM .
BIOCHEMICAL PHARMACOLOGY, 1974, 23 (14) :2057-2059
[10]   FACTORS INVOLVED IN UPTAKE OF CORTICOSTERONE BY RAT-LIVER CELLS [J].
RAO, ML ;
RAO, GS ;
ECKEL, J ;
BREUER, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 500 (02) :322-332