EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-1 IN THE SUBMANDIBULAR-GLAND OF THE RAT

被引：27

作者：

AMANO, O ^{[1
]}

TSUJI, T ^{[1
]}

NAKAMURA, T ^{[1
]}

ISEKI, S ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1991年 / 39卷 / 12期

关键词：

TRANSFORMING GROWTH FACTOR-BETA-1; IMMUNOCYTOCHEMISTRY; INSITU HYBRIDIZATION; SUBMANDIBULAR GLAND; RAT;

D O I：

10.1177/39.12.1940322

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We employed immunocytochemical and in situ hybridization techniques to study the expression of transforming growth factor beta-1 (TGF-beta-1) in rat submandibular gland. Immunoreactivity for TGF-beta-1 was observed in the cells of granular convoluted tubules (GCTs), striated ducts, and excretory ducts, whereas it was absent in the intercalated ducts and secretory acini in both male and female rats. Immunoelectron microscopy revealed the ultrastructural localization of TGF-beta-1 in the secretory granules of GCT cells. On the other hand, signals for rat TGF-beta-1 mRNA were abundant in the GCT and striated duct cells but were lacking in the excretory duct cells. These results provided evidence for the production of TGF-beta-1 in the GCTs and striated ducts of rat submandibular gland.

引用

页码：1707 / 1711

页数：5

共 29 条

[1] BIOLOGICALLY-ACTIVE POLYPEPTIDES IN SUB-MANDIBULAR GLANDS
BARKA, T
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1980, 28 (08) : 836 - 859
[2] REGULATION OF INTESTINAL EPITHELIAL-CELL GROWTH BY TRANSFORMING GROWTH-FACTOR TYPE-BETA
BARNARD, JA
BEAUCHAMP, RD
COFFEY, RJ
MOSES, HL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) : 1578 - 1582
[3] TRANSFORMING GROWTH FACTOR-BETA MESSENGER-RNA INCREASES DURING LIVER-REGENERATION - A POSSIBLE PARACRINE MECHANISM OF GROWTH-REGULATION
BRAUN, L
MEAD, JE
PANZICA, M
MIKUMO, R
BELL, GI
FAUSTO, N
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (05) : 1539 - 1543
[4] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE
CHIRGWIN, JM
PRZYBYLA, AE
MACDONALD, RJ
RUTTER, WJ
[J]. BIOCHEMISTRY, 1979, 18 (24) : 5294 - 5299
[5] DERYNCK R, 1986, J BIOL CHEM, V261, P4377
[6] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
[7] TRANSFORMING GROWTH FACTOR-BETA-1 - HISTOCHEMICAL-LOCALIZATION WITH ANTIBODIES TO DIFFERENT EPITOPES
FLANDERS, KC
THOMPSON, NL
CISSEL, DS
VANOBBERGHENSCHILLING, E
BAKER, CC
KASS, ME
ELLINGSWORTH, LR
ROBERTS, AB
SPORN, MB
[J]. JOURNAL OF CELL BIOLOGY, 1989, 108 (02) : 653 - 660
[8] GATHERER D, 1990, DEVELOPMENT, V110, P445
[9] EPIDERMAL GROWTH FACTOR-LIKE MATERIAL IN RAT SUB-MANDIBULAR GLAND
GRESIK, EW
VANDERNOEN, H
BARKA, T
[J]. AMERICAN JOURNAL OF ANATOMY, 1979, 156 (01): : 83 - 89
[10] ROLE OF TRANSFORMING GROWTH FACTOR-BETA IN THE DEVELOPMENT OF THE MOUSE EMBRYO
HEINE, UI
MUNOZ, EF
FLANDERS, KC
ELLINGSWORTH, LR
LAM, HYP
THOMPSON, NL
ROBERTS, AB
SPORN, MB
[J]. JOURNAL OF CELL BIOLOGY, 1987, 105 (06) : 2861 - 2876

← 1 2 3 →