CLINICAL-APPLICATION OF THE POLYMERASE CHAIN-REACTION FOR A RAPID DIAGNOSIS OF MYCOBACTERIUM-TUBERCULOSIS INFECTION

被引：6

作者：

KIKUCHI, Y

OKA, S

KIMURA, S

MITAMURA, K

SHIMADA, K

机构：

[1] Department of Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo

来源：

INTERNAL MEDICINE | 1992年 / 31卷 / 08期

关键词：

GENE AMPLIFICATION; 65KD ANTIGEN; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; LABORATORY DIAGNOSIS; CLINICAL USE;

D O I：

10.2169/internalmedicine.31.1016

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A gene amplification method of Mycobacterium tuberculosis DNA by the polymerase chain reaction (PCR) has been devised. A primer pair used in this study is 5'GTTGCCGTGGCGG TATCGG3' and 5'GCGACATTACGGGGCAGGTGG3', which brackets a 152-base region encoding the 65KD antigen, and a specific probe is 5'TTTGGGGTCATCTTTGGAGCG3'. The procedure could be completed within 2 days. The specificity and the sensitivity of the PCR for M. tuberculosis complex in identifying M. tuberculosis complex did not conflict with the conventional methods at all. Using this method, we could diagnose three cases of the disease, which had been very difficult to diagnose by the conventional methods, by detecting the DNA from the blood, liver biopsy specimen, lung aspirate, and pleural effusion.

引用

页码：1016 / 1022

页数：7

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