DETERMINATION OF A CEFUROXIME AND AMINOPHYLLINE/THEOPHYLLINE MIXTURE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high-performance liquid chromatographic method for the simultaneous determination of cefuroxime and aminophylline/theophylline has been developed. Cefuroxime was separated from aminophylline/theophylline within 8 min using a octadecylsilane column and a mobile phase consisting of a 10:1 mixture of 0.1 M acetate buffer pH 3.4 - acetonitrile at ambient temperature. Orcinol was used as internal standard and the analytes were monitored at 254 nm. The method was free of interference from degradation products related to any of the analytes. Drug to internal standard;,peak area ratios were linear (r(2) greater than or equal to 0.9998) for each analyte with good precision (RSD less than or equal to 2.1%)and accuracy (less than or equal to 2.3%).