DETECTION OF HEPATITIS-C VIRUS-RNA IN SERUM, BY COMBINING REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION IN ONE TUBE

被引：7

作者：

TILSTON, P

CORBITT, G

机构：

[1] North Manchester Virus Laboratory, Booth Hall Children's Hospital

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 44卷 / 01期

关键词：

HEPATITIS-C VIRUS; HCV; PCR; COMBINED RT PCR;

D O I：

10.1016/0166-0934(93)90007-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reverse transcription of viral RNA into cDNA and its subsequent amplification by polymerase chain reaction (PCR) are generally carried out as two separate reactions. Here we describe a novel method for the detection of HCV RNA in serum combining both steps in one reaction tube using a wax interface to separate the two sets of reactants during initial cDNA synthesis. This enabled optimisation of both reactions, simplified the test and thereby reduced the risk of cross-contamination.

引用

收藏

页码：57 / 65

页数：9

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