D(1) DOPAMINE-RECEPTOR BINDING AND MESSENGER-RNA LEVELS ARE NOT ALTERED AFTER NEONATAL 6-HYDROXYDOPAMINE TREATMENT - EVIDENCE AGAINST DOPAMINE-MEDIATED INDUCTION OF D(1) DOPAMINE-RECEPTORS DURING POSTNATAL-DEVELOPMENT

被引:30
作者
DUNCAN, GE
BREESE, GR
CRISWELL, HE
JOHNSON, KB
SCHAMBRA, UB
MUELLER, RA
CARON, MG
FREMEAU, RT
机构
[1] UNIV N CAROLINA, SCH MED, DEPT PSYCHIAT, CHAPEL HILL, NC 27514 USA
[2] UNIV N CAROLINA, SCH MED, DEPT CELL BIOL & ANAT, CHAPEL HILL, NC 27514 USA
[3] DUKE UNIV, MED CTR, HOWARD HUGHES MED INST LABS, DURHAM, NC 27710 USA
[4] DUKE UNIV, MED CTR, DEPT PHARMACOL, DURHAM, NC 27710 USA
[5] DUKE UNIV, MED CTR, DEPT NEUROBIOL, DURHAM, NC 27710 USA
[6] DUKE UNIV, MED CTR, DEPT CELL BIOL, DURHAM, NC 27710 USA
[7] DUKE UNIV, MED CTR, DEPT MED, DURHAM, NC 27710 USA
关键词
D(1) DOPAMINE RECEPTORS; 6-HYDROXYDOPAMINE; RECEPTOR BINDING; IN-SITU HYBRIDIZATION; DEVELOPMENT; SCH-23390; ONTOGENY;
D O I
10.1111/j.1471-4159.1993.tb13616.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of dopaminergic innervation on the postnatal developmental expression of D1 dopamine receptors was investigated. Bilateral destruction of dopamine-containing neurons was achieved by treating rats intracisternally with 6-hydroxydopamine (6-OHDA) on postnatal day 3, and rats were killed on day 21. To ensure effective reduction of D1 receptor activation by residual dopamine, a group of 6-OHDA-lesioned rats was given twice daily injections of the D1 receptor antagonist SCH-23390, from day 4 to 20. D1 dopamine receptor binding was assessed in the caudate-putamen, nucleus accumbens, and olfactory tubercle by quantitative autoradiographic analysis of [H-3]SCH-23390 binding. In addition, the relative amount of D1A receptor mRNA was assessed by in situ hybridization of a S-35-labeled riboprobe. In the developing rats, neither the amount of [H-3]SCH-23390 binding nor the amount of D1A receptor mRNA was altered by 6-OHDA lesioning followed by chronic treatment with SCH-23390. Thus, bilateral destruction of dopamine-containing neurons and treatment with SCH-23390 in neonatal rats did not interfere with the developmental expression of D1 receptors or alter the levels of mRNA that code for this receptor protein. Treatment of intact rats with SCH-23390 from postnatal day 4 to 20 also did not alter [H-3]SCH-23390 binding or levels of D1 receptor mRNA. However, adult rats treated chronically with SCH-23390 exhibited increased [H-3]SCH-23390 binding but did not show a significant change in D1 receptor mRNA levels.
引用
收藏
页码:1255 / 1262
页数:8
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