MOLECULAR-CLONING OF CDNA-ENCODING MOUSE CDC21 AND CDC46 HOMOLOGS AND CHARACTERIZATION OF THE PRODUCTS - PHYSICAL INTERACTION BETWEEN P1(MCM3) AND CDC46 PROTEINS

被引：71

作者：

KIMURA, H ^{[1
]}

TAKIZAWA, N ^{[1
]}

NOZAKI, N ^{[1
]}

SUGIMOTO, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC SCI,DEPT CHEM 2,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 12期

关键词：

D O I：

10.1093/nar/23.12.2097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two new mouse genes encoding proteins that belong to the yeast minichromosome maintenance (MCM) protein family, which is involved in the initiation of DNA replication, were isolated and their nucleotide sequence was determined. They were a putative CDC46/MCM5 homolog and a putative cdc21 homolog. About 30% amino acid identity was obtained between members in the family, and >40% between the putative mouse and yeast homologs. The expression of these genes was cell-cycle specific at the late G(1) to S phase. Immunochemical analyses showed the physical interaction between mouse P1MCM3 and CDC46 protein. These results suggest that MCM proteins function in co-ordination for DNA replication.

引用

页码：2097 / 2104

页数：8

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