BIAMPEROMETRIC DETERMINATION OF GLYCEROL AND TRIGLYCERIDES WITH OPEN TUBULAR CARBON ELECTRODES IN FLOW STREAMS

Biamperometric measurements, with open tubular carbon electrodes in flowing streams, are used to monitor the concentration of hexacyanoferrate(II) produced by the diaphorasecatalyzed reaction between NADH and hexacyanoferrate(III). NADH produced by the glycerol dehydrogenase-catalyzed reaction between glycerol and NAD can be measured in this manner to determine glycerol concentrations; linear calibration plots are obtained in the range 5 × 10-5-6 × 10-1 M glycerol. Triglycerides are hydrolyzed by lipase and the glycerol liberated is measured similarly. Glycerol and triglycerides are determined in serum control samples by the standard addition technique. © 1979.