A NOVEL ASSOCIATION BETWEEN AN ENDEMIC STICKLEBACK AND A PARASITIC DINOFLAGELLATE .2. MORPHOLOGY AND LIFE-CYCLE

An unusual dinoflagellate has been discovered in association with an endemic population of stickleback, Gasterosteus (L.), from the Queen Charlotte Islands, Canada. The dinoflagellate spends most of its life cycle as a coccoid vegetative cyst, not as a parasitic trophont. The vegetative cyst is unique in containing a rigid fenestrated matrix, which is penetrated by cytoplasmic processes that emanate from a central area containing the dinokaryotic nucleus and associated chlorplasts. Some pores in the matrix are filled by oil droplets or starch granules. Intracellular bacteria are found throughout the cyst, sometimes in association with the nucleus. The cytoplasm contains accumulation bodies, microbodies, polyhedral crystals, chloroplasts and polyvesicular bodies. The encysted dinoflagellate has several potential strategies. It can 1) shed its wall and become amoeboid; 2) undergo sporogenesis and give rise to both regular and resistant spores; 3) divide mitotically, with a gradual reduction in the size of daughter cells down to 20-mu-m; and 4) apparently form a resting cyst, during which it secretes a thick outer wall composed of five layers. Taxonomically, this unusual dinoflagellate appears to be a new member of the Blastodiniales, although its position will become clearer when details of the motile stage are known.