TRISOMY AND CHROMOSOME SIZE CHANGES IN HYBRID TRYPANOSOMES FROM A GENETIC CROSS BETWEEN TRYPANOSOMA-BRUCEI-RHODESIENSE AND TRYPANOSOMA-BRUCEI-BRUCEI

被引：37

作者：

GIBSON, W ^{[1
]}

GARSIDE, L ^{[1
]}

BAILEY, M ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH VET,DEPT VET MED,BRISTOL BS18 7DU,AVON,ENGLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 51卷 / 02期

基金：

英国医学研究理事会;

关键词：

TRYPANOSOME; TRYPANOSOMA-BRUCEI; GENETIC EXCHANGE; CHROMOSOME; TRISOMY;

D O I：

10.1016/0166-6851(92)90069-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Further analysis of hybrid clones from an experimental cross of Trypanosoma brucei rhodesiense 058 and T. b. brucei 196 shows 2 of the hybrid clones to have DNA contents about 1.5 times parental values. This represents over 40000 kb of extra DNA. Comparison of the molecular karyotypes of parental and progeny trypanosomes shows that the bulk of the extra DNA constitutes chromosomes greater than 1 Mb in size, although a small proportion can be accounted for by an increased number of mini-chromosomes. The 2 hybrid clones have 3 alleles at several loci for housekeeping genes as shown by RFLP and isoenzyme analysis. Trisomy of the chromosome carrying phosphoglycerate kinase and tubulin genes and that carrying the phospholipase C gene was demonstrated by analysis of molecular karyotypes. These chromosomes appear prone to substantial size alterations associated with genetic exchange. Our results for one of the hybrid clones are completely consistent with it being triploid and the product of fusion of haploid and diploid nuclei.

引用

页码：189 / 199

页数：11

共 31 条

[1] MOLECULAR-CLONING AND ANALYSIS OF 2 TANDEMLY LINKED GENES FOR PYRUVATE-KINASE OF TRYPANOSOMA-BRUCEI [J].

ALLERT, S ;

ERNEST, I ;

POLISZCZAK, A ;

OPPERDOES, FR ;

MICHELS, PAM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (01) :19-27

[2] ON THE DNA CONTENT AND PLOIDY OF TRYPANOSOMES [J].

BORST, P ;

VANDERPLOEG, M ;

VANHOEK, JFM ;

TAS, J ;

JAMES, J .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1982, 6 (01) :13-23

[3] NEW CULTURE MEDIUM FOR MAINTENANCE OF TSETSE TISSUES AND GROWTH OF TRYPANOSOMATIDS [J].

CUNNINGHAM, I .

JOURNAL OF PROTOZOOLOGY, 1977, 24 (02) :325-329

[4] MANY TRYPANOSOME MESSENGER-RNAS SHARE A COMMON 5' TERMINAL SEQUENCE [J].

DELANGE, T ;

MICHELS, PAM ;

VEERMAN, HJG ;

CORNELISSEN, AWCA ;

BORST, P .

NUCLEIC ACIDS RESEARCH, 1984, 12 (09) :3777-3790

[5] GENETIC EXCHANGE IN TRYPANOSOMA-BRUCEI-BRUCEI - VARIABLE CHROMOSOMAL LOCATION OF HOUSEKEEPING GENES IN DIFFERENT TRYPANOSOME STOCKS [J].

GIBSON, W ;

GARSIDE, L .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 45 (01) :77-90

[6] ANALYSIS OF A GENETIC CROSS BETWEEN TRYPANOSOMA-BRUCEI-RHODESIENSE AND TRYPANOSOMA-BRUCEI-BRUCEI [J].

GIBSON, WC .

PARASITOLOGY, 1989, 99 :391-402

[7] SPECIES-SPECIFIC DNA PROBES FOR THE IDENTIFICATION OF AFRICAN TRYPANOSOMES IN TSETSE FLIES [J].

GIBSON, WC ;

DUKES, P ;

GASHUMBA, JK .

PARASITOLOGY, 1988, 97 :63-73

[8] SIZE-FRACTIONATION OF THE SMALL CHROMOSOMES OF TRYPANOZOON AND NANNOMONAS TRYPANOSOMES BY PULSED FIELD GRADIENT GEL-ELECTROPHORESIS [J].

GIBSON, WC ;

BORST, P .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1986, 18 (02) :127-140

[9] CHROMOSOME ORGANIZATION OF THE PROTOZOAN TRYPANOSOMA-BRUCEI [J].

GOTTESDIENER, K ;

GARCIAANOVEROS, J ;

LEE, MG ;

VANDERPLOEG, LHT .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (11) :6079-6083

[10] HYBRID FORMATION BETWEEN AFRICAN TRYPANOSOMES DURING CYCLICAL TRANSMISSION [J].

JENNI, L ;

MARTI, S ;

SCHWEIZER, J ;

BETSCHART, B ;

LEPAGE, RWF ;

WELLS, JM ;

TAIT, A ;

PAINDAVOINE, P ;

PAYS, E ;

STEINERT, M .

NATURE, 1986, 322 (6075) :173-175

← 1 2 3 4 →