TGF-BETA STIMULATES RAT MESANGIAL CELL-PROLIFERATION IN CULTURE - ROLE OF PDGF BETA-RECEPTOR EXPRESSION

被引：85

作者：

HABERSTROH, U ^{[1
]}

ZAHNER, G ^{[1
]}

DISSER, M ^{[1
]}

THAISS, F ^{[1
]}

WOLF, G ^{[1
]}

STAHL, RAK ^{[1
]}

机构：

[1] UNIV FRANKFURT,DEPT MED,DIV NEPHROL,THEODOR STERN KAI 7,W-6000 FRANKFURT 70,GERMANY

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 02期

关键词：

TRANSFORMING GROWTH FACTOR-BETA; MESANGIAL CELLS; PLATELET-DERIVED GROWTH FACTOR;

D O I：

10.1152/ajprenal.1993.264.2.F199

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Transforming growth factor (TGF)-beta is known to increase mesangial cell (MC) matrix; however, its possible role on MC proliferation is controversial. We therefore studied the influence of TGF-beta on MC proliferation in culture and evaluated its effect on the platelet-derived growth factor (PDGF) B-chain as well as the expression of the PDGF beta-receptor. TGF-beta (1 ng/ml) increases MC DNA synthesis by approximately threefold after 48 h of incubation. TGF-beta-induced MC proliferation was also confirmed by cell counts. A neutralizing anti-TGF-beta antibody completely blocked this growth promoting activity. The levels of PDGF beta-receptor steady-state mRNA were increased by TGF-beta at 48 h. This was associated with an increase in receptor density per cell as measured by receptor kinetic studies. PDGF B-chain mRNA was also increased by TGF-beta at 48 h. A neutralizing anti-PDGF B-antibody causes no reduction of TGF-beta-induced DNA synthesis; however, suramin completely inhibited the mitogenic effect of TGF-beta. We conclude that TGF-beta stimulates MC growth in long-term culture, a process in which upregulation of the PDGF beta-receptor and enhanced synthesis of PDGF B-chain might be involved.

引用

页码：F199 / F205

页数：7

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