SELECTION OF ANTICOAGULANTS FOR LYMPHOCYTE IMMUNOPHENOTYPING - EFFECT OF SPECIMEN AGE ON RESULTS

被引：25

作者：

NICHOLSON, JKA

GREEN, TA

BEATRICE, S

CALVELLI, T

DENNY, T

INHORN, S

KIDD, P

LANDAY, A

MANDY, F

PARKER, J

PAXTON, H

机构：

[1] US DEPT HHS,PUBL HLTH SERV,CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV HIV AIDS,ATLANTA,GA 30333

[2] US DEPT HHS,PUBL HLTH SERV,CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV HIV AIDS,ATLANTA,GA 30333

[3] STATE LAB HYG,MADISON,WI

[4] UNIV WASHINGTON,SEATTLE,WA 98195

[5] RUSH PRESBYTERIAN ST LUKES MED CTR,CHICAGO,IL 60612

[6] UNIV SO CALIF,SCH MED,LOS ANGELES,CA 90033

[7] NEW YORK CITY DEPT PUBL HLTH,NEW YORK,NY

[8] UMD,NEW JERSEY MED SCH,NEWARK,NJ

[9] BUR LAB & RES SERV,FED CTR AIDS,OTTAWA,ON,CANADA

[10] MARYLAND MED RES INST,BALTIMORE,MD

[11] YESHIVA UNIV ALBERT EINSTEIN COLL MED,BRONX,NY 10461

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 165卷 / 01期

关键词：

ANTICOAGULANT; FLOW CYTOMETRY; HIV INFECTION; IMMUNOPHENOTYPING;

D O I：

10.1016/0022-1759(93)90103-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In a multi-center study, whole blood specimens from 31 HIV-positive and 43 HIV-negative donors were collected in three different anticoagulants and assayed for lymphocyte subsets fresh (within 6 h), and 1 and 2 days later. Each center prepared the specimens by their routine whole blood lysis procedure, labeling with a recommended panel of two-color monoclonal antibody combinations. 1 day (up to about 30 h) after blood collection, the results obtained from blood collected in EDTA (ethylenediamine tetra-acetate), ACD (acid citrate dextrose), and heparin were similar to fresh. Up to 48 h, only ACD and heparin, not EDTA, yielded results similar to fresh specimens. These results were similar for both HIV-positive and -negative specimens.

引用

页码：31 / 35

页数：5

共 10 条

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