AGE-DEPENDENT INCREASES OF IMMUNOREACTIVE IMIDAZOLINE RECEPTORS IN THE HUMAN BRAIN - POSSIBLE ASSOCIATION OF A 29/30-KDA PROTEIN WITH THE I-2-IMIDAZOLINE RECEPTOR IDENTIFIED BY [H-3] IDAZOXAN

被引：23

作者：

GARCIASEVILLA, JA

SASTRE, M

ESCRIBA, PV

机构：

[1] Laboratory of Neuropharmacology, Department of Fundamental Biology and Health Sciences, University of the Balearic Islands, E-07071 Palma de Mallorca

来源：

NEUROSCIENCE LETTERS | 1995年 / 184卷 / 02期

关键词：

IMIDAZOLINE RECEPTOR PROTEINS; WESTERN IMMUNE BLOT; I-2-IMIDAZOLINE RECEPTOR; H-3] IDAZOXAN BINDING; HUMAN BRAIN;

D O I：

10.1016/0304-3940(94)11188-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In the postmortem human brain (15 specimens of frontal cortex), the immunodetection and quantification of imidazoline receptor (IR) proteins (29/30 and 47 kDa) and the specific binding of [H-3]idazoxan to I-2-IR were determined in parallel to study the effect of aging (range 3-89 years) on these proteins and its possible association. The immunoreactivity of the 29/30 kDa IR protein, similar to the I-2-IR, showed a significant positive correlation with the age of the subjects at death (r = 0.54; P = 0.04). Moreover, the levels of this Ik protein also correlated significantly with the density of I-2-IR in the brain of the same subjects (r = 0.67; P = 0.01). It is suggested that the I-2-IR labeled by [H-3]idazoxan in the human brain is related to the 29/30 kDa IR protein identified by immunoblot analysis.

引用

页码：133 / 136

页数：4

共 12 条

[1] CDNA CLONING OF HUMAN-LIVER MONOAMINE OXIDASE-A AND OXIDASE-B - MOLECULAR-BASIS OF DIFFERENCES IN ENZYMATIC-PROPERTIES [J].

BACH, AWJ ;

LAN, NC ;

JOHNSON, DL ;

ABELL, CW ;

BEMBENEK, ME ;

KWAN, SW ;

SEEBURG, PH ;

SHIH, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4934-4938

[2] IMIDAZOLINE BINDING-SITES IN HUMAN PLACENTA - EVIDENCE FOR HETEROGENEITY AND A SEARCH FOR PHYSIOLOGICAL-FUNCTION [J].

DIAMANT, S ;

ELDARGEVA, T ;

ATLAS, D .

BRITISH JOURNAL OF PHARMACOLOGY, 1992, 106 (01) :101-108

[3] IMMUNODETECTION OF PUTATIVE IMIDAZOLINE RECEPTOR PROTEINS IN THE HUMAN AND RAT-BRAIN AND OTHER TISSUES [J].

ESCRIBA, PV ;

SASTRE, M ;

WANG, H ;

REGUNATHAN, S ;

REIS, DJ ;

GARCIASEVILLA, JA .

NEUROSCIENCE LETTERS, 1994, 178 (01) :81-84

[4]

GARCIASEVILLA JA, 1994, IN PRESS ANN NY ACAD

[5]

MICHEL MC, 1992, TRENDS PHARMACOL SCI, V13, P369

[6]

MIRALLES A, 1993, J PHARMACOL EXP THER, V264, P1187

[7] OPPOSITE AGE-DEPENDENT CHANGES OF ALPHA(2A)-ADRENOCEPTORS AND NONADRENOCEPTOR [H-3] IDAZOXAN BINDING-SITES (I(2)-IMIDAZOLINE SITES) IN THE HUMAN BRAIN - STRONG CORRELATION OF I(2) WITH MONOAMINE OXIDASE-B SITES [J].

SASTRE, M ;

GARCIASEVILLA, JA .

JOURNAL OF NEUROCHEMISTRY, 1993, 61 (03) :881-889

[8]

SASTRE M, 1994, J PHARMACOL EXP THER, V269, P1062

[9] IDENTIFICATION OF AN IMIDAZOLINE-GUANIDINIUM RECEPTIVE SITE IN MITOCHONDRIA FROM RABBIT CEREBRAL-CORTEX [J].

TESSON, F ;

PARINI, A .

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1991, 208 (01) :81-83

[10]

TESSON F, 1991, J BIOL CHEM, V266, P155

← 1 2 →